ZFIN ID: ZDB-FIG-070615-5
Ma et al., 2007 - Jagged2a-notch signaling mediates cell fate choice in the zebrafish pronephric duct. PLoS Genetics   3(1):e18 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-5

Fig. S3 notch1a, jagged2a, and her9 Are Expressed in the Distal Duct at the Time of Cell-Fate Determination

(A and B) Fluorescent double in situ hybridization of notch1a and myoD [93] revealed that notch1a is expressed in the pronephric duct spanning from somite 10 to 14 (arrows) at 18 ss.

(C and D) Fluorescent double in situ hybridization of jagged2a and myoD revealed that mosaic jagged2a expression is found in the pronephric duct spanning from somite 8 to 14 (arrows) at 22 ss.

(E and F) Fluorescent double in situ hybridization of jagged2a and myoD revealed that jagged2a-expressing single cells are found in the pronephric duct spanning from somite 8 to 14 (arrows) at 24 hpf.

(G and H) Fluorescent double in situ hybridization of her9 and myoD revealed that her9 is expressed in the pronephric duct spanning from somite 10 to 12 (arrows) at 18 ss.

(I and J) Fluorescent double in situ hybridization of jagged2a (green), slc4a2/ae2 (red, anterior), and ret1 (red, posterior) revealed that jagged2a-expressing single cells are found in the distal duct between the proximal duct (marked by slc4a2/ae2; [27]) and the cloaca (marked by ret1; [11]). Small arrows demarcate the jagged2a-expressing single cell domain, arrowheads demarcate the slc4a2/ae2-expressing domain, and big arrows demarcate the ret1-expressing domain.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
her9 WT standard conditions 14-19 somites pronephric duct ISH
jag2b WT standard conditions 20-25 somites to Prim-5 pronephric duct ISH
myod1 WT standard conditions 14-19 somites somite ISH
20-25 somites to Prim-5 somite ISH
notch1a WT standard conditions 14-19 somites pronephric duct ISH
ret WT standard conditions Prim-5 pronephric duct ISH
slc4a2a WT standard conditions Prim-5 pronephric duct ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.