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ZIRC
ZFIN ID: ZDB-FIG-070314-11
Kuan et al., 2007 - Neuropilin asymmetry mediates a left-right difference in habenular connectivity. Development (Cambridge, England)   134(5):857-865 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Long-pec to Day 4

Fig. 2 Asymmetric expression of nrp1a in the dorsal diencephalon. (A,B) Left-sided expression of nrp1a (black arrow) is detected by 2 days (A) and increases by 4 days (B). (C) nrp1a expression (red) overlaps with the dense neuropil of the left habenula revealed by anti-acetylated tubulin immunolabeling (green) at 4 days. (D,E) The nrp1a-expressing neurons (red) are a subset of the Lov-immunoreactive population (D, green) and are also distinct from Ron-immunoreactive cells (E, green) of the left habenula (arrowheads). (F-H) Expression of (F) nrp1b, (G) nrp2a and (H) nrp2b was not detected in the habenular nuclei. (I,J) Laterality of nrp1a expression (orange) correlates with parapineal position (otx5 expression, black arrowheads) in (I) mock-injected (94±2% left bias, n=89) and (J) spaw MO-injected 4-day-old larvae (56±2% sinistral and 44±2% dextral, n=96). A-C and F-J are dorsal views of the brain; C and D are lateral views of composite Z-stack confocal images through the left habenula. Left (L) and right (R) sides of the brain are indicated. Scale bars: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
kctd12.1 AB standard conditions Day 4 habenula IHC
kctd12.2 AB standard conditions Day 4 habenula IHC
nrp1a AB control Day 4 habenula ISH
AB standard conditions Long-pec diencephalon ISH
Day 4 diencephalon ISH
Day 4 habenula ISH
AB + MO2-spaw standard conditions Day 4 habenula ISH
nrp1b AB standard conditions Long-pec Not Detected habenula ISH
nrp2a AB standard conditions Long-pec Not Detected habenula ISH
nrp2b AB standard conditions Long-pec Not Detected habenula ISH
otx5 AB control Day 4 parapineal organ ISH
AB + MO2-spaw standard conditions Day 4 parapineal organ ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development