ZFIN ID: ZDB-FIG-070117-7
Jeong et al., 2007 - Patterning the zebrafish diencephalon by the conserved zinc-finger protein Fezl. Development (Cambridge, England)   134(1):127-136 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-15

Fig. 3 The fezl morphants exhibit anterior expansion of the ZLI. (A-D) Marked anterior expansion of shh-expressing ZLI (B,D) was observed in fezl morphants. Arrows in A,B indicate the floor plate; arrowhead in A,B indicates hypothalamus; square brackets indicate the ZLI. (E,F) Double labeling of foxg1 (a telencephalic marker) and shh, showing the expression of shh in the prethalamic area in the fezl morphant. (G,H) Anterior expansion of foxa2 expression in the fezl morphant. Arrow in H indicates the expansion of foxa2-expressing cells; square brackets indicate the ZLI. (I,J) Expanded expression of dbx1a near the ZLI (arrow) and in the thalamus (arrowhead) in the fezl morphant. (K-P) dbx1a, shh and irx3a expression in the 16-somite-stage (16s) control and fezl morphants. Arrows in I-L indicate lhx5-expressing cells in the posterior tubercular region, and in M-N indicate the floor plate; arrowhead in I,J indicates lhx5-expressing cells in the thalamus, and in M-P indicates expanding shh expression in the presumptive ZLI; square bracket in M indicates the ZLI; dotted line in O,P indicates the anterior boundary of irx3a expression in the posterior diencephalon and midbrain region. All are lateral views of embryonic brains, except C,D, which are dorsal views. t, thalamus; hy, hypothalamus; mb, midbrain; ptc, pretectum; tel, telencephalon; fp, floor plate; bp, basal plate.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dbx1a WT standard conditions 14-19 somites diencephalon ISH
Prim-15 diencephalon ISH
WT + MO1-fezf2 standard conditions 14-19 somites diencephalon ISH
Prim-15 diencephalon ISH
foxa2 WT standard conditions Prim-5 zona limitans intrathalamica ISH
WT + MO1-fezf2 standard conditions Prim-5 zona limitans intrathalamica ISH
foxg1a WT standard conditions Prim-15 telencephalon ISH
WT + MO1-fezf2 standard conditions Prim-15 telencephalon ISH
irx3a WT standard conditions 14-19 somites diencephalon ISH
14-19 somites midbrain ISH
WT + MO1-fezf2 standard conditions 14-19 somites diencephalon ISH
14-19 somites midbrain ISH
shha WT standard conditions 14-19 somites diencephalon ISH
14-19 somites floor plate ISH
Prim-5 basal plate midbrain region ISH
Prim-5 floor plate ISH
Prim-5 hypothalamus ISH
Prim-5 zona limitans intrathalamica ISH
Prim-15 floor plate ISH
Prim-15 hypothalamus ISH
Prim-15 tegmentum ISH
Prim-15 zona limitans intrathalamica ISH
WT + MO1-fezf2 standard conditions 14-19 somites diencephalon ISH
14-19 somites floor plate ISH
Prim-5 basal plate midbrain region ISH
Prim-5 floor plate ISH
Prim-5 hypothalamus ISH
Prim-5 zona limitans intrathalamica ISH
Prim-15 floor plate ISH
Prim-15 hypothalamus ISH
Prim-15 tegmentum ISH
Prim-15 zona limitans intrathalamica ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development