ZFIN ID: ZDB-FIG-060809-14
Nissen et al., 2006 - A zebrafish screen for craniofacial mutants identifies wdr68 as a highly conserved gene required for Endothelin-1 expression. BMC Developmental Biology   6:28 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 20-25 somites to Prim-25

Fig. 5 wdr68 acts upstream of the edn1 pathway. A) In situ hybridization (ISH) with dlx2 on 12 somites stage embryos. Wild type and dys/dys mutant animals were indistinguishable. B) ISH on 20 somites stage wild type sibling animal with edn1 probe. C) dys/dys homozygotes display reduced edn1 expression at 20 somites stage. D) ISH on 28 hpf wild type sibling animal with edn1 probe. E) dys/dys homozygotes display reduced edn1 expression at 28 hpf. F) ISH on 41 hpf wild type sibling with bapx1 probe. G) dys/dys homozygotes display reduced bapx1 expression. H, I, J, K, L, M, N, O) Red arrowhead indicates 1st arch expression domain, blue arrowhead indicates 2nd arch expression domain. H) ISH on 28 hpf wild type sibling with hand2 probe. I) dys/dys homozygotes display reduced 1st arch hand2 expression, red arrowhead. Red dotted outline indicates expression in developing heart. J) dys+wdr68MO animals display reduced hand2 expression in both 1st and 2nd arches, red and blue arrowheads, respectively. K) edn1-MO animals lack hand2 expression in both the 1st and 2nd arches. L, M, N, O) Red asterisk indicates olfactory expression domain. Black asterisk indicates ear expression domain. L) ISH on 28 hpf wild type sibling with dlx3b probe. M) dys/dys homozygotes display reduced 1st arch dlx3b expression, red arrowhead. N) dys+wdr68MO animals display reduced dlx3b expression in both 1st and 2nd arches, red and blue arrowheads, respectively. O) edn1-MO animals lack dlx3b expression in both the 1st and 2nd arches. P, Q, R, S) Red underline indicates 1st arch region for sox9a expression. Black underline indicates 2nd arch region for sox9a expression. P) ISH with sox9a probe on 28 hpf wild type sibling. Q) Reduction of 1st arch sox9a expression without significant effect on 2nd arch sox9a expression in dys/dys animals. R) Injecting dys animals with wdr68MO caused further reduction of 1st arch sox9a expression without substantial effects on 2nd arch sox9a expression. S) edn1-MO animals show reduced but still detectable sox9a expression in both the 1st and 2nd arches.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dlx3b WT standard conditions Prim-5 olfactory placode ISH
Prim-5 otic vesicle ISH
Prim-5 pharyngeal arch ISH
WT + MO1-edn1 standard conditions Prim-5 olfactory placode ISH
Prim-5 otic vesicle ISH
Prim-5 Not Detected pharyngeal arch ISH
dcaf7hi3812Tg/hi3812Tg standard conditions Prim-5 olfactory placode ISH
Prim-5 otic vesicle ISH
Prim-5 pharyngeal arch ISH
dcaf7hi3812Tg/hi3812Tg + MO1-dcaf7 standard conditions Prim-5 olfactory placode ISH
Prim-5 otic vesicle ISH
Prim-5 pharyngeal arch ISH
edn1 WT standard conditions 20-25 somites anatomical structure ISH
Prim-5 pharyngeal arch ISH
dcaf7hi3812Tg/hi3812Tg standard conditions 20-25 somites anatomical structure ISH
Prim-5 pharyngeal arch ISH
hand2 WT standard conditions Prim-5 pharyngeal arch ISH
Prim-5 primitive heart tube ISH
WT + MO1-edn1 standard conditions Prim-5 Not Detected pharyngeal arch ISH
Prim-5 primitive heart tube ISH
dcaf7hi3812Tg/hi3812Tg standard conditions Prim-5 pharyngeal arch ISH
Prim-5 primitive heart tube ISH
dcaf7hi3812Tg/hi3812Tg + MO1-dcaf7 standard conditions Prim-5 pharyngeal arch ISH
Prim-5 primitive heart tube ISH
nkx3-2 WT standard conditions Prim-25 pharyngeal arch ISH
dcaf7hi3812Tg/hi3812Tg standard conditions Prim-25 pharyngeal arch ISH
sox9a WT standard conditions Prim-5 pharyngeal arch ISH
WT + MO1-edn1 standard conditions Prim-5 pharyngeal arch ISH
dcaf7hi3812Tg/hi3812Tg standard conditions Prim-5 pharyngeal arch ISH
dcaf7hi3812Tg/hi3812Tg + MO1-dcaf7 standard conditions Prim-5 pharyngeal arch ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.