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Fig. S3 Expression profile of iro2a and iro1b. All prim-5 brains are lateral view, anterior to the left. Expression of iro2a (A-D) and iro1b (E-H) in wild type (A,E), OtxH (B,F), fgf8–/– (C,G) and fgf8–/–; OtxH (D,H) embryos. Both genes are expressed in diencephalon subdomains, midbrain (mb), r1 and hindbrain in wild type. Diencephalic and midbrain domains are, respectively, reduced or lacking in OtxH embryos. A fainter midbrain domain is expanded in fgf8–/– embryos. Prospective cerebellar territories are iros-positive in wild type, OtxH and fgf8–/–; OtxH embryos (arrows), while OtxH embryos might have a general slight decrease in mid/hinbrain staining.