etsrp Is Required for vegf and scl Signaling (A–D) Etsrp is required for Vegf signaling as assayed for flk1 expression at 26 hpf. (A) Control uninjected embryo, (B) vegf RNA-injected embryo, (C) 7.5 ng of etsrp MO2-injected embryo, (D) vegf RNA- and etsrp MO2-co-injected embryo. Note that vegf RNA induces strong flk1 expression in (B) while vegf RNA and etsrp MO co-injection results in loss of flk1 expression in (D), similar to the etsrp morphant phenotype in (C). (E,F) Etsrp expression analysis in Vegf morphants at 26 hpf. (E) Control uninjected embryo; (F) 10.5 ng of vegf MO-injected embryo. Note that vegf morphants have lost etsrp expression in the intersegmental vessels (arrowhead, E). (G–J) Scl knockdown affects gata1 but not etsrp expression at the 15-somite stage. Dorsal view, anterior is to the left. (G,I) Control uninjected embryo; (H,J) 10 ng scl UTR-MO-injected embryo. (G,H) gata1 expression; (I,J) etsrp expression. (K–N) Etsrp is required for scl signaling in clo mutants as analyzed for flk1 expression at the 15-somite stage. (K) Control uninjected embryo; (L) 7.5 ng etsrp MO2-injected embryo; (M) scl RNA-injected embryo; (N) scl RNA- and etsrp MO2-co-injected embryo. Note that scl RNA causes ectopic flk1 expression in (M) which is lost upon knockdown of Etsrp in (N).
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