Molecular Analysis of Vascular Endothelial and Hematopoietic Markers in etsrp Morphants (A, C, E, G, I, K, M, O, Q, S) Uninjected control embryo; (D, H, J, L, P, R, T) 8 ng etsrp MO2-injected embryo; (B, F, N) 12 ng etsrp MO1+MO2 (1:1) mix-injected embryos for the maximum knockdown. All embryos are at 24 hpf, except as noted. Scale bar, 0.2 mm. (A,B) flk1 expression; (C,D) admr expression; (E,F) cdh5 expression; (G,H) dusp5 expression; (I,J) flt4 expression; (K,L) crl expression. Note that the vascular expression of the markers in (A–L) is almost absent in etsrp morphants. (M,N) fli1 expression; (O,P) etsrp expression. Note the more intense etsrp expression and reduced fli1 expression in angioblasts which remain dispersed and fail to coalesce into blood vessels in etsrp morphants. Inset, (P) DIC image of scattered angioblasts in etsrp morphants. (Q,R) scl expression, 22 hpf. Note that scl expression appears unaffected at this stage except for more intense staining in a subset of head vessels in etsrp morphants (arrowhead). (S,T) gata1 expression, 22 somites. Note that no significant difference in hematopoietic gata1 expression is observed between the control embryos and etsrp morphants.
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