ZFIN ID: ZDB-FIG-060120-4
Cha et al., 2006 - Cyclooxygenase-1-derived PGE2 promotes cell motility via the G-protein-coupled EP4 receptor during vertebrate gastrulation. Genes and Development   20(1):77-86 Full text @ Genes Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Shield to Bud
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage Range: Shield to Prim-5

Fig. 3 EP4-deficient embryos exhibit convergence and extension defect during gastrulation. (A,B,D,E) Lateral view, dorsal to the right. (C,F,G,H) Lateral view, anterior to the left. (I,K) Animal view, dorsal to the right. (J,L) Dorsal view, animal pole to the top. (M,O) Dorsal view, anterior to the top. (N,P) Anterior view, dorsal to the bottom. (M,N) Boundaries indicate the width of the notochord. (N,P) Boundaries indicate the distance from the posterior end of the forebrain to the anterior end of the notochord. (A-C,I,J,M,N) Uninjected control. (D-F,H,K,L,O,P) ep4-MO2 (2 ng) injected embryos. (G,H) ep4 RNA (80 pg) injected embryos. (H) Embryos injected with ep4-MO2 (2 ng) + ep4 RNA (80 pg). (I,K) bmp4 expressions. (J,L) gsc expressions. (M,O) no tail (ntl) expressions. (N,P) six3, pax2, and ntl expressions. (h) Head; (tb) tailbud; (D) dorsal; (fb) forebrain; (mh) mid-hindbrain; (no) notochord. The arrowhead points to the location of the prechordal plate at the tailbud stage. The white star highlights the blastopore to illustrate delayed epiboly. The black star indicates the animal pole.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
bmp4 WT standard conditions Shield presumptive mesoderm ISH
WT + MO2-ptger4a standard conditions Shield presumptive mesoderm ISH
gsc WT standard conditions Shield shield ISH
WT + MO2-ptger4a standard conditions Shield shield ISH
pax2a WT standard conditions Bud midbrain hindbrain boundary neural plate ISH
WT + MO2-ptger4a standard conditions Bud midbrain hindbrain boundary neural plate ISH
six3b WT standard conditions Bud neuroectoderm ISH
WT + MO2-ptger4a standard conditions Bud neuroectoderm ISH
tbxta WT standard conditions Bud axial mesoderm ISH
WT + MO2-ptger4a standard conditions Bud axial mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-ptger4a standard conditions Shield axis decreased length, abnormal
Shield epiboly involved in gastrulation with mouth forming second delayed, abnormal
1-4 somites axis decreased length, abnormal
Bud axis decreased length, abnormal
1-4 somites convergent extension involved in gastrulation delayed, abnormal
Bud convergent extension involved in gastrulation delayed, abnormal
1-4 somites head position, abnormal
Bud head position, abnormal
Bud notochord increased width, abnormal
Prim-5 convergent extension involved in gastrulation disrupted, abnormal
Prim-5 notochord undulate, abnormal
Prim-5 somite elongated, abnormal
Prim-5 whole organism anterior-posterior axis decreased length, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Genes and Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Genes Dev.