FIGURE

Fig. 3

ID

ZDB-FIG-060120-4

Publication

Cha et al., 2006 - Cyclooxygenase-1-derived PGE2 promotes cell motility via the G-protein-coupled EP4 receptor during vertebrate gastrulation

Other Figures

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Fig. 3

EP4-deficient embryos exhibit convergence and extension defect during gastrulation. (A,B,D,E) Lateral view, dorsal to the right. (C,F,G,H) Lateral view, anterior to the left. (I,K) Animal view, dorsal to the right. (J,L) Dorsal view, animal pole to the top. (M,O) Dorsal view, anterior to the top. (N,P) Anterior view, dorsal to the bottom. (M,N) Boundaries indicate the width of the notochord. (N,P) Boundaries indicate the distance from the posterior end of the forebrain to the anterior end of the notochord. (A-C,I,J,M,N) Uninjected control. (D-F,H,K,L,O,P) ep4-MO2 (2 ng) injected embryos. (G,H) ep4 RNA (80 pg) injected embryos. (H) Embryos injected with ep4-MO2 (2 ng) + ep4 RNA (80 pg). (I,K) bmp4 expressions. (J,L) gsc expressions. (M,O) no tail (ntl) expressions. (N,P) six3, pax2, and ntl expressions. (h) Head; (tb) tailbud; (D) dorsal; (fb) forebrain; (mh) mid-hindbrain; (no) notochord. The arrowhead points to the location of the prechordal plate at the tailbud stage. The white star highlights the blastopore to illustrate delayed epiboly. The black star indicates the animal pole.

Expression Data

Genes:	bmp4 gsc pax2a six3b tbxta
Fish:	WT WT + MO2-ptger4a
Knockdown Reagent:	MO2-ptger4a
Anatomical Terms:	axial mesoderm midbrain hindbrain boundary neural plate neuroectoderm presumptive mesoderm shield
Stage Range:	Shield to Bud

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO2-ptger4a
Knockdown Reagent:	MO2-ptger4a
Observed In:	axis convergent extension involved in gastrulation epiboly involved in gastrulation with mouth forming second head notochord somite whole organism anterior-posterior axis
Stage Range:	Shield to Prim-5

Phenotype Detail

Acknowledgments

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