ZFIN ID: ZDB-FIG-060120-3
Cha et al., 2006 - Cyclooxygenase-1-derived PGE2 promotes cell motility via the G-protein-coupled EP4 receptor during vertebrate gastrulation. Genes and Development   20(1):77-86 Full text @ Genes Dev.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage: Bud
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Bud

Fig. 2 Ptges-deficient embryos exhibit gastrulation defects that are suppressed by coincubation with PGE2. (A-H) Lateral view, dorsal to the right. (I-K) Animal view, dorsal to the bottom. (L-Q) Dorsal view, anterior to the top. (R,S) Lateral view, anterior to the left. (I-K) Boundaries indicate the distance from the posterior end of the forebrain to the anterior end of the notochord. (L-Q) Boundaries indicate the width of the notochord. (A,I,L,O) Control embryos treated with DMSO. (B,F-H,J,K,M,N,S) Embryos injected with 2 ng of ptges-MO2. (E,P,Q) Embryos injected with 4 ng of ptges-MO2. (C,D) Embryos injected with 10 ng of cox1-MO. (D) Embryos coinjected with cox1-MO (10 ng) and cox1 RNA (50 pg). (F) Embryos injected with 2 ng of ptges-MO2 + PGI2. (G) Embryos injected with 2 ng of ptges-MO2 + PGF. (H,K,N) Embryos injected with 2 ng of ptges-MO2 + PGE2. (Q) Embryos injected with 4 ng of ptges-MO2 + PGE2. (H) Embryos coinjected with ptges-MO2 (2 ng) + mouse ptges RNA (100 pg). (I-K) Expression patterns of six3b, pax2.1. (L-Q) Expression pattern of no tail. (T) Quantitative analyses of the distance between the forebrain (fb) and notochord (no) in DMSO, ptges-MO2, and ptges-MO2 + PGE2 (n = 10 per each group). (U) Quantitative analyses of width of ntl expression in DMSO, ptges-MO2, and ptges-MO2 + PGE2 (n = 10 per each group). (fb) Forebrain; (mh) mid-hindbrain; (no) notochord. The white star marks the blastopore to illustrate delayed epiboly. For details see text.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
pax2a WT control Bud midbrain hindbrain boundary neural plate ISH
WT + MO2-ptges chemical treatment: prostaglandin E2 Bud midbrain hindbrain boundary neural plate ISH
six3b WT control Bud neuroectoderm ISH
WT + MO2-ptges chemical treatment: prostaglandin E2 Bud neuroectoderm ISH
WT + MO2-ptges standard conditions Bud neuroectoderm ISH
tbxta WT control Bud axial mesoderm ISH
WT + MO2-ptges chemical treatment: prostaglandin E2 Bud axial mesoderm ISH
WT + MO2-ptges chemical treatment: prostaglandin E2 Bud axial mesoderm ISH
WT + MO2-ptges standard conditions Bud axial mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO2-ptges standard conditions Bud axis decreased length, abnormal
Bud convergent extension involved in gastrulation delayed, abnormal
Bud epiboly involved in gastrulation with mouth forming second arrested, abnormal
Bud epiboly involved in gastrulation with mouth forming second delayed, abnormal
Bud head position, abnormal
Bud notochord increased width, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Genes and Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Genes Dev.