header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-051123-6
Norton et al., 2005 - HSPG synthesis by zebrafish Ext2 and Extl3 is required for Fgf10 signalling during limb development. Development (Cambridge, England)   132(22):4963-4973 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Long-pec

Fig. 6 Expression of fin marker genes compared in extl3/box and ext2/dak mutants. In situ hybridisation staining of wild-type (A,D,G,J), extl3/box (B,E,H,K) and ext2/dak (C,F,I,L) embryos. All panels are dorsal views with anterior towards the left. At 48 hpf, extl3/box mutant embryos have reduced expression of all markers analysed (B,E,H,K) when compared with wild type (A,D,G,J). ext2/dak mutants have a stronger phenotype. There is a strong reduction of dlx2a (C), fgf8 (F) and eng1a (L). Expression of shh is absent in ext2/dak (I) by 48 hpf of development.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dlx2a WT standard conditions Long-pec pectoral fin ISH
ext2tw25e/tw25e standard conditions Long-pec pectoral fin ISH
extl3tw24/tw24 standard conditions Long-pec pectoral fin ISH
en1a WT standard conditions Long-pec pectoral fin ISH
ext2tw25e/tw25e standard conditions Long-pec pectoral fin ISH
extl3tw24/tw24 standard conditions Long-pec pectoral fin ISH
fgf8a WT standard conditions Long-pec pectoral fin ISH
ext2tw25e/tw25e standard conditions Long-pec pectoral fin ISH
extl3tw24/tw24 standard conditions Long-pec pectoral fin ISH
shha WT standard conditions Long-pec pectoral fin ISH
ext2tw25e/tw25e standard conditions Long-pec Not Detected pectoral fin ISH
extl3tw24/tw24 standard conditions Long-pec pectoral fin ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development