FIGURE

Fig. 5

ID

ZDB-FIG-051102-5

Publication

Kaji et al., 2004 - dlx3b and dlx4b function in the development of Rohon-Beard sensory neurons and trigeminal placode in the zebrafish neurula

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Fig. 5

Double in situ hybridization of HuC/isl-1 and neural/mesodermal markers in MOs-injected embryos. (A, C, E, G, I, K, M, O) Wild-type embryos at 3-somite stage. (B, D, F, H, J, L, N, P) 20 ng ATG-dlx3b-MO/20 ng ATG-dlx4b-MO-injected embryos at 3-somite stage. (A and B) Expression of HuC (blue) and krx20/pax2a, a marker of rhombomeres 3 and 5 and midbrain–hindbrain boundary (red). (C and D) Expression of isl-1 (blue) and krx20/pax2a (red). (E and F) Expression of HuC (blue) and axial marking floor plate (red). (G and H) Expression of isl-1 (blue) and axial (red). (I and J) Expression of HuC (blue) and gsc/ntl expressed in the prechordal plate and notochord (red). (K and L) Expression of isl-1 (blue) and gsc/ntl (red). (M and N) Expression of HuC (blue) and paraxis showing expression in the somites (blue). Numbers 1–3 correspond to the somites. (O and P) Expression of isl-1 (blue) and paraxis (blue). Numbers 1–3 correspond to the somites. In (B, D, J, L), neurons in the trigeminal placodes (arrowhead) are highly reduced or absent, shown by the expression of HuC and isl-1. The expression of HuC and isl-1 in the RB neurons (F, H, N, P; arrow) are highly reduced or absent. However, no changes are observed in neural/mesodermal marker expression in dlx3b and dlx4b MOs-injected embryos (B, D, F, H, J, L, N, P).

Expression Data

Genes:	egr2b elavl3 foxa2 gsc isl1a pax2a tbxta tcf15
Fish:	AB/TL AB/TL + MO2-dlx4b + MO3-dlx3b
Knockdown Reagents:	MO2-dlx4b MO3-dlx3b
Anatomical Terms:	midbrain hindbrain boundary neural keel notochord prechordal plate presumptive floor plate presumptive rhombomere 3 presumptive rhombomere 5 primary motor neuron Rohon-Beard neuron segmental plate somite
Stage:	1-4 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB/TL + MO2-dlx4b + MO3-dlx3b
Knockdown Reagents:	MO2-dlx4b MO3-dlx3b
Observed In:	Rohon-Beard neuron trigeminal ganglion neuron
Stage:	1-4 somites

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 276(2), Kaji, T., and Artinger, K.B., dlx3b and dlx4b function in the development of Rohon-Beard sensory neurons and trigeminal placode in the zebrafish neurula, 523-540, Copyright (2004) with permission from Elsevier. Full text @ Dev. Biol.