ZFIN ID: ZDB-FIG-050930-14
Babb et al., 2005 - Zebrafish R-cadherin (Cdh4) controls visual system development and differentiation. Developmental dynamics : an official publication of the American Association of Anatomists   233(3):930-945 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 6 Expression of early markers of photoreceptor identity is reduced in cdh4 morphants. A-F: Zebrafish embryos were injected with either standard control morpholino oligonucleotide (MO, A,D) or cdh4 MO (B,C,E,F). crx (A-C) and otx5 (D-F) labeling in 78 hours postfertilization (hpf), embryos were used as markers of photoreceptor specification and differentiation. A,D: In controls, crx and otx5 labeling were confined to the photoreceptor layer and the outermost part of the inner nuclear layer. B,E: Some reduction of staining was seen in slightly affected cdh4 morphants. C,F: In severe cdh4 morphants, both crx and otx5 staining were reduced and lacked defined laminae.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
crx WT standard conditions Protruding-mouth retinal inner nuclear layer ISH
Protruding-mouth retinal photoreceptor layer ISH
WT + MO1-cdh4 standard conditions Protruding-mouth retinal inner nuclear layer ISH
Protruding-mouth retinal photoreceptor layer ISH
otx5 WT standard conditions Protruding-mouth retinal inner nuclear layer ISH
Protruding-mouth retinal photoreceptor layer ISH
WT + MO1-cdh4 standard conditions Protruding-mouth retinal inner nuclear layer ISH
Protruding-mouth retinal photoreceptor layer ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-cdh4 standard conditions Protruding-mouth photoreceptor cell differentiation disrupted, abnormal
Protruding-mouth retina layer formation disrupted, abnormal
WT + MO2-cdh4 standard conditions Protruding-mouth photoreceptor cell differentiation disrupted, abnormal
Protruding-mouth retina layer formation disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.