ZFIN ID: ZDB-FIG-050930-13
Babb et al., 2005 - Zebrafish R-cadherin (Cdh4) controls visual system development and differentiation. Developmental dynamics : an official publication of the American Association of Anatomists   233(3):930-945 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Day 4

Fig. 5 cdh4 knockdown affects differentiation of the retina. A-T: Zebrafish embryos were injected with either standard control morpholino oligonucleotide (MO, A,E,I,M,Q) or cdh4 MO (B-D,F-H,J-L,N-P,R-T). Retinal ganglion cells were visualized at 2 days postfertilization (dpf), using confocal imaging of zn5-immunostained whole-mount embryos. Images are ventral view projections at the level of the optic nerve. Zn5 identifies retinal ganglion cells and their axon processes, among other cells in the developing zebrafish. A: In control embryos, the retinal ganglion cell layer was uniform in thickness and had smooth boundaries. B: The retinal ganglion cell layer was often expanded in cdh4 MO-injected embryos that were only slightly affected. C: In moderately affected embryos, the retinal ganglion cell layer was sparse with irregular boundaries and the optic nerve was thin. D: In severely affected cdh4 knockdowns (D), the retinal ganglion cell layer ranged from nearly absent (D), with only one or two zn5-positive cells and their projections, to altogether absent (not shown). E-T: For Pax6 and zpr-1 immunolocalization, embryos were fixed at 3 dpf (E-L) or at 4 dpf (M-T) and 8 μm horizontal cryosections were cut. Adjacent sections of the same eye (except H, L) were immunostained for Pax6-positive amacrine cells (E-H,M-P) and zpr-1-positive double cone photoreceptors (I-L,Q-T) and visualized by confocal microscopy. cdh4 knockdown images are arranged from left to right as a graded series of slightly affected (F,J,N,R), moderately affected (G,K,O,S), then severely affected (H,L,P,T) embryos. F,H,J,L,N-P,R-T: Amacrine cells and photoreceptor cells fail to differentiate in severe knockdowns (H,L,P,T), but, when present in less affected knockdowns (F,J,N,O,R,S), they were localized in appropriate retinal laminae. Scale bar = 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
alcama WT standard conditions Long-pec retinal ganglion cell layer IHC
WT + MO1-cdh4 standard conditions Long-pec retinal ganglion cell layer IHC
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-cdh4 standard conditions Long-pec cranial nerve II decreased thickness, abnormal
Long-pec retina development in camera-type eye disrupted, abnormal
Long-pec retinal ganglion cell decreased amount, abnormal
Long-pec retinal ganglion cell layer compartment boundary rough, abnormal
Long-pec retinal ganglion cell layer decreased thickness, abnormal
Long-pec retinal ganglion cell layer sparse, abnormal
Protruding-mouth amacrine cell differentiation disrupted, abnormal
Protruding-mouth photoreceptor cell differentiation disrupted, abnormal
Protruding-mouth retina lacks parts or has fewer parts of type amacrine cell, abnormal
Protruding-mouth retina lacks parts or has fewer parts of type photoreceptor cell, abnormal
Day 4 amacrine cell differentiation disrupted, abnormal
Day 4 photoreceptor cell differentiation disrupted, abnormal
Day 4 retina lacks parts or has fewer parts of type amacrine cell, abnormal
Day 4 retina lacks parts or has fewer parts of type photoreceptor cell, abnormal
WT + MO2-cdh4 standard conditions Long-pec cranial nerve II decreased thickness, abnormal
Long-pec retina development in camera-type eye disrupted, abnormal
Long-pec retinal ganglion cell decreased amount, abnormal
Long-pec retinal ganglion cell layer compartment boundary rough, abnormal
Long-pec retinal ganglion cell layer decreased thickness, abnormal
Long-pec retinal ganglion cell layer sparse, abnormal
Protruding-mouth amacrine cell differentiation disrupted, abnormal
Protruding-mouth photoreceptor cell differentiation disrupted, abnormal
Protruding-mouth retina lacks parts or has fewer parts of type amacrine cell, abnormal
Protruding-mouth retina lacks parts or has fewer parts of type photoreceptor cell, abnormal
Day 4 amacrine cell differentiation disrupted, abnormal
Day 4 photoreceptor cell differentiation disrupted, abnormal
Day 4 retina lacks parts or has fewer parts of type amacrine cell, abnormal
Day 4 retina lacks parts or has fewer parts of type photoreceptor cell, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.