ZFIN ID: ZDB-FIG-050930-11
Babb et al., 2005 - Zebrafish R-cadherin (Cdh4) controls visual system development and differentiation. Developmental dynamics : an official publication of the American Association of Anatomists   233(3):930-945 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 3 Reduction of Cdh4 protein levels correlates with phenotypic severity of cdh4 morphants. A-D: Zebrafish embryos were injected with either standard control morpholino oligonucleotide (MO, A) or cdh4 MO (B-D). A: Confocal imaging of 3 days postfertilization (dpf) Cdh4 immunostained cryosections showed Cdh4 localization in the retinal ganglion cell layer, in the amacrine cell layer of the inner nuclear layer, in the lens, and in the optic nerve of the control embryo. B,C: In slightly and moderately affected cdh4 MO-injected embryos, staining of the retinal ganglion cell layer, the amacrine cell layer and the optic nerve was reduced. D: In severely affected embryos, Cdh4 staining was not detected in the neural retina and reduced in the lens (the apparent immunoreactivity of the ectoderm and lens is nonspecific, that is, it could not be competed with excess immunizing peptide; Liu et al.,[2001b]). E: Corresponding immunoblots of individual embryos of various phenotypic severities in the hypomorphic series showed that, compared with control embryos at 3 dpf (con), Cdh4 expression was reduced in slightly and moderately affected embryos (sli, mod) and absent in severely affected embryos (sev).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
cdh4 Ab1-cdh4 WT standard conditions Protruding-mouth amacrine cell IHC
Ab1-cdh4 Protruding-mouth cranial nerve II IHC
Ab1-cdh4 Protruding-mouth retinal ganglion cell layer IHC
Ab1-cdh4 Protruding-mouth whole organism WB
Ab1-cdh4 WT + MO1-cdh4 standard conditions Protruding-mouth Not Detected amacrine cell IHC
Ab1-cdh4 Protruding-mouth Not Detected cranial nerve II IHC
Ab1-cdh4 Protruding-mouth Not Detected retinal ganglion cell layer IHC
Ab1-cdh4 Protruding-mouth Not Detected whole organism WB
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-cdh4 standard conditions Protruding-mouth eye decreased size, abnormal
Protruding-mouth lens decreased size, abnormal
WT + MO2-cdh4 standard conditions Protruding-mouth eye decreased size, abnormal
Protruding-mouth lens decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.