FIGURE

Fig. 1

ID

ZDB-FIG-050715-5

Publication

Rhinn et al., 2005 - Positioning of the midbrain-hindbrain boundary organizer through global posteriorization of the neuroectoderm mediated by Wnt8 signaling

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Fig. 1

Expression of MHB markers is normal in mutants with defects in notochord formation and in shield-ablated embryos. (A-D) In situ hybridization and Ntl immunostaining (brown) of control embryos, (E-H) ntl mutant embryos, (I-L) flh mutant embryos and (M-P) shield-ablated embryos at tailbud stage. Probes were applied: otx2 (A,E,I,M), gbx1 (B,F,J,N), eng2 (C,G,K,O), fgf8 (D,H,L,P). In B', no Ntl immunostaining has been performed. No changes in the AP position of the different MHB markers analyzed is observed in ntl mutants, in flh mutants or in shield-ablated embryos when compared with wild-type embryos. (D,P) Brackets indicate the reduced midline width following shield ablation. (A-O) Lateral views, anterior upwards; (D,H,L,P,B',F',N') dorsal views, anterior upwards.

Expression Data

Genes:	en2a fgf8a gbx1 otx2b tbxta
Fish:	WT noto^tk241/tk241 tbxta^tc41/tc41
Anatomical Terms:	hindbrain neural plate midbrain hindbrain boundary neural keel midbrain neural keel notochord
Stage:	1-4 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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