FIGURE

Fig. S1

ID

ZDB-FIG-050721-1

Publication

Rhinn et al., 2005 - Positioning of the midbrain-hindbrain boundary organizer through global posteriorization of the neuroectoderm mediated by Wnt8 signaling

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Fig. S1

Non-neural marker and pan-neural marker expression in Wnt8 gain-of-function experiments. Overexpression of wnt8 suppresses non-neural markers throughout the epiblast: (A) Control embryo and (B) injected embryos with wnt8 RNA 100 pg stained for p63 at 60% of epibloy. (C) Control embryo and (D) embryo injected with wnt8 RNA 100 pg stained for foxi1 at 60% of epibloy. Overexpression of wnt8 induces ectopic expression of neural markers throughout the epiblast. (E) Control embryo and (F) embryos injected with wnt8 RNA 100 pg stained for zic2.2 at 60% of epibloy. (G) Control embryo and (H) embryos injected with wnt8 RNA 100 pg stained for sox31 at 60% of epibloy. See also Fig. 6 for a similar analysis carried out using clones of wnt8 misexpressing cells.

Expression Data

Genes:	foxi1 sox19b tp63 zic2b
Fish:	WT
Anatomical Term:	epiblast
Stage:	Shield

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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