Fig. 6

Patterning of hindbrain is affected in the gir mutant. Expression of krox20 and myoD (A and B), valentino and myoD (C and D), pax2.1 and krox20 (E and F), gbx2 and krox20 (G and H), eph-b2 (I and J), and otx2 (K and L) in wild type (A, C, E, G, I, and K) and gir mutant (B, D, F, H, J, and L) embryos. (A–D) The distance between the rhombomere 5 (krox20) or 6 (valentino) and the first somite is expanded, and the expression domain of valentino is reduced, in the gir mutant at the 14-somite stage. (E and F) The distance between midbrain–hindbrain boundary (pax2.1) and rhombomere 3 (krox20) is reduced in the gir mutant at the 14-somite stage. (G and H) The distance between rhombomere 1 (gbx2) and rhombomere 3 (krox20) is slightly reduced in the gir mutant at the 10-somite stage. (I and J) The distance between rhombomere 7 and the first somite is expanded in the gir mutant at the 12-somite stage. (K and L) The otx2 expression domain in the midbrain was reduced in the gir mutant at the 10-somite stage. Dorsal views (A, B, G, and H) and lateral views (C–F and I–L) are shown. Genotypes were determined by PCR assay after the photographs had been taken. r, rhombomere; s, somites.