Fig. 2

Patterning defects in the pectoral fin bud and tailbud in the gir mutant. (A and B) Morphology of the pectoral fin bud in wild type (A) and gir mutant (B) embryos at 36 hpf. The arrowhead indicates the fin bud (A). The gir mutant embryo has no fin bud (B). (C and D) Dorsal views of the wild type (C) and gir mutant (D) embryos at 80 hpf. The arrows indicate pectoral fins. No or small fins were observed in the gir mutant embryo (D). (E–L) Expression of tbx5.1 (E–H), shh (I and J), and ntl (K and L) was examined by in situ hybridization in wild type (E, G, I, and K) and gir mutant (F, H, J, and L) embryos. The expression domain of tbx5.1 has shifted anteriorly in the gir mutant at 20 hpf (F) and 28 hpf (H). The fin bud expression of shh (shown by arrows in I) is absent in the gir mutant at 28 hpf (J). ntl expression is reduced in the tailbud of the gir mutant at the 7-somite stage (L). Genotypes were determined by PCR assay after the photographs had been taken in E, F, K, and L.