FIGURE 3

FIGURE 3

SAL promotes the recruitment of innate immune cells to the wound and infection sites. (A) Images of whole body SB staining of 3-dpf embryos. (B) Fluorescent images of 3-dpf Tg (mpeg1:loxP-GFP) embryos. A and B Scale bar: 200 μm. (C) Statistics of the number of SB⁺ neutrophils in A. (D) Quantification of total macrophage fluorescent area in B. (E) Schematic view of tail amputation experiment and the tissue for RNA extraction (upper panel); the recruitment of SB⁺ neutrophils to wound (left panel); and the recruitment of mpeg1:loxp:GFP⁺ macrophages to wound (right panel). Scale bar: 50 μm. (F) Statistics of the number of SB⁺ neutrophils in E. (G) Statistics of the number of macrophages in E. (H) Expression of various cytokines in the tail region at 2 hpa. (I) Expression of macrophage and neutrophil recruitment-related chemokines in the tail region at 2 hpa. (J) Images of the SB⁺ neutrophils (left panel) and mpeg1:LRLG⁺ macrophages (right panel) infiltration to infection sites. Scale bar: 50 μm. (K) Statistics of the number of SB⁺ neutrophils in J. (L) Statistics of the number of macrophages in J. A–G, J–L: n = 20 per group; H and I: n = 30 per sample. This is one representative data from three independent biological replicates.