ZFIN Image: North et al., 2009, Fig. 2

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Figure Caption

Fig. 2 A Beating Heart Is Required for HSC Formation and Artery Development

(A–H) Effect of sih mutation on HSC and vascular formation at 36 hpf.

(A and E) runx1/cmyb expression is greatly reduced in sih^-/- embryos compared to WT siblings.

(B and F) flk1 expression reveals a grossly normal vascular pattern in sih^-/- embryos; changes in the development of the intersomitic vessels and vascular plexus were noted in some animals.

(C and F) ephB2 expression is diminished in sih^-/- embryos.

(D and H) flt4 expression is expanded in sih^-/- embryos.

(I) The expression levels of HSC (runx1, cmyb), vascular (flk), and arterial (ephB2) markers are significantly decreased in sih^-/- embryos compared to sibling controls (mean ± SD, t test, p < 0.05, n = 3), as measured by qPCR at 36 hpf.

(J and K) The sih mutation has no effect on primitive hematopoiesis as seen by benzidine staining at 36 hpf; in the absence of a heartbeat blood is pooled in the major vessels.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

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Reprinted from Cell, 137(4), North, T.E., Goessling, W., Peeters, M., Li, P., Ceol, C., Lord, A.M., Weber, G.J., Harris, J., Cutting, C.C., Huang, P., Dzierzak, E., and Zon, L.I., Hematopoietic stem cell development is dependent on blood flow, 736-748, Copyright (2009) with permission from Elsevier. Full text @ Cell