ZFIN Image: Pei et al., 2009, Fig. 4

Image

Figure Caption

Fig. 4 Tissue on both sides of midline bifurcations acquires midline identity. (A) Whole-mount in situ analysis of gene expression. WT and MZsqt embryos were heat treated overnight (22 h) starting at the 64-cell stage and fixed on 1 day post fertilization for whole-mount in situ analysis. All embryos are oriented with a dorsal view, anterior on the left. (B–C) Histological sections of WT and MB embryos. WT and MZsqt embryos were heat treated for 22 h starting at the 64-cell stage and then reared for 2 more days, fixed with 4% paraformaldehyde, paraffin embedded, sectioned (5 μm/section) and stained with hematoxylin and eosin. The approximate section sites for the WT embryo (B) and the MZsqt embryo (C) are indicated. Apostrophes (') indicate the sections from the same specimen. Arrows indicate neural tubes. Arrowheads indicate guts.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 326(1), Pei, W., and Feldman, B., Identification of common and unique modifiers of zebrafish midline bifurcation and cyclopia, 201-211, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.