FIGURE

Fig. 8

ID

ZDB-FIG-240528-27

Publication

Tuttle et al., 2024 - Single-cell analysis of Rohon-Beard neurons implicates Fgf signaling in axon maintenance and cell survival

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Fig. 8

Fgf inhibition and dovitinib treatment induce Wallerian-like axon degeneration and RB neuron apoptosis. A–C, Stills from time-lapse movies of larval RB:GFP caudal tails treated with DMSO (A), SU5402 (B), or dovitinib (C). Yellow arrowheads = maintained axon terminals, magenta arrowheads = degenerating axons. D–F, Stills from time lapse movies of larval RB:GFP dorsal neurons demonstrating RB apoptosis. Yellow arrowheads = surviving RB neurons, magenta arrowheads = RB neurons undergoing apoptosis. G–I, Lateral view of RB:GFP dorsal neurons immunostained for cleaved Caspase-3 to mark apoptosis (yellow arrowheads). Compared to DMSO-treated larvae (G), SU5402 (H), and dovitinib (I). Scale bars = 50 μm. I, treatment displayed Cas3+ RB neurons. J, Quantification of RB neuron apoptosis detected by Cas3+ immunostaining. DMSO = 0 ± 0.2 RB neurons, dovitinib = 1.2 ± 0.2, SU5402 = 2.6 ± 0, analyzed by one-way ANOVA with post hoc Tukey's test (F = 07.81, p = 0.0013). Scale bars = 50 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J. Neurosci.