FIGURE

Fig. 7

ID

ZDB-FIG-231113-3

Publication

Hagedorn et al., 2023 - Transcription factor induction of vascular blood stem cell niches in vivo

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Fig. 7

Ectopic vascular regions recruit HSPCs and support their proliferation

(A) WISH for runx1 shows HSPC localization in a control (left) and 3-factor injected embryo (right). Black arrowheads denote endogenous CHT localization; and red arrows point to ectopic localization.

(B) Image shows runx1:mCherry⁺ HSPCs localized outside the CHT within a large ectopic region of mrc1a 1.3kb:GFP expression in an embryo injected with a pool of ubi:ETV2, SOX7, and Nr2f2. Magnification of boxed area is shown on the right.

(C) ECs ectopically expressing mrc1a 1.3kb:GFP are associated with cxcl12a:dsRed2⁺ stromal cells, similar to ECs in the CHT. Asterisk denotes notochord expression of cxcl12a:dsRed2, and the black arrowhead points to CHT localization. Magnification of boxed region is shown.

(D) Time-lapse series shows a runx1:mCherry⁺ HSPC (red arrows) initially arriving at an ectopic site and subsequently dividing (images show magnification of region with red arrow in Video S8). Time is shown as hh:mm. Control and experimental groups were blinded prior to scoring and all experiments were performed at least three times, with independent clutches. Scale bars represent 100 μm in (A-C), and 30 μm in (D).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Cell, 58(12), Hagedorn, E.J., Perlin, J.R., Freeman, R.J., Wattrus, S.J., Han, T., Mao, C., Kim, J.W., Fernández-Maestre, I., Daily, M.L., D'Amato, C., Fairchild, M.J., Riquelme, R., Li, B., Ragoonanan, D.A.V.E., Enkhbayar, K., Henault, E.L., Wang, H.G., Redfield, S.E., Collins, S.H., Lichtig, A., Yang, S., Zhou, Y., Kunar, B., Gomez-Salinero, J.M., Dinh, T.T., Pan, J., Holler, K., Feldman, H.A., Butcher, E.C., van Oudenaarden, A., Rafii, S., Junker, J.P., Zon, L.I., Transcription factor induction of vascular blood stem cell niches in vivo, 1037-1051.e4, Copyright (2023) with permission from Elsevier. Full text @ Dev. Cell