Fig. 6
- ID
- ZDB-FIG-231113-2
- Publication
- Hagedorn et al., 2023 - Transcription factor induction of vascular blood stem cell niches in vivo
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3-factor induction of ectopic niche EC gene expression (A) Images show WISH for mrc1a in a control embryo (top) or after injection of a 3-factor pool containing ETV2, SOX7 and Nr2f2 (middle) or ETS1, SOX7, and Nr2f2 (bottom). Black arrowheads point to endogenous expression while red arrows point to ectopic expression in all panels of this figure. (B) Graph reports quantification of the percentage of injected embryos that displayed ectopic mrc1a WISH staining after transcription factor overexpression. Chi-squared test and Fisher’s exact test for pairwise comparisons with the Holm step-down process to correct for multiple comparisons; ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001. (C) Images show WISH for CHT niche EC genes in control embryos (left) and embryos injected with the 3-factor ubi:ETV2, SOX7, and Nr2f2 combination (right). (D) Images show ectopic GFP expression in vessels in the anterior head and yolk region of mrc1a 125bp:GFP transgenic embryos injected with the 3-factor ubi:ETV2, SOX7, and Nr2f2 mixtures. (E) Images show ectopic expression in mrc1a 125bp:GFP; kdrl:mCherry double-positive embryos that were injected with muscle-specific mylz2:ETV2, SOX7, Nr2f2 (left), or endothelial-specific nrp1b:ETV2, SOX7, and Nr2f2 (right) plasmids. Magnification of boxed regions is shown at the bottom. Control and experimental groups were blinded prior to scoring and all experiments were performed at least three times, with independent clutches. Scale bars in (C) and (E) represent 100 μm. |
Reprinted from Developmental Cell, 58(12), Hagedorn, E.J., Perlin, J.R., Freeman, R.J., Wattrus, S.J., Han, T., Mao, C., Kim, J.W., Fernández-Maestre, I., Daily, M.L., D'Amato, C., Fairchild, M.J., Riquelme, R., Li, B., Ragoonanan, D.A.V.E., Enkhbayar, K., Henault, E.L., Wang, H.G., Redfield, S.E., Collins, S.H., Lichtig, A., Yang, S., Zhou, Y., Kunar, B., Gomez-Salinero, J.M., Dinh, T.T., Pan, J., Holler, K., Feldman, H.A., Butcher, E.C., van Oudenaarden, A., Rafii, S., Junker, J.P., Zon, L.I., Transcription factor induction of vascular blood stem cell niches in vivo, 1037-1051.e4, Copyright (2023) with permission from Elsevier. Full text @ Dev. Cell