Fig. 5
- ID
- ZDB-FIG-231113-1
- Publication
- Hagedorn et al., 2023 - Transcription factor induction of vascular blood stem cell niches in vivo
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7-factor induction of ectopic niche EC gene expression (A) Schematic depicts the strategy used in transcription factor overexpression experiments. (B and C) Images show embryos that were injected with control DNA (left) or a pool of seven transcription factors (right) from the Ets, Sox, and NHR families (FLI1, ETV2, ETS1, SOX7, Sox18, Nr2f2, and RXRA) and then stained by WISH for mrc1a (B) or sele (C). Red arrows denote regions of ectopic expression and black arrowheads point to normal domains of expression in all panels of this figure. (D) Images show mrc1a 1.3kb:GFP; kdrl:mCherry double transgenic embryos that were injected with control DNA (left) or a pool of seven transcription factors (right). (E) Images show ectopic expression of the mrc1a 1.3kb:GFP and kdrl:mCherry transgenes over the yolk extension in a 7-factor injected embryo. Magnification of the boxed area is shown at the right. Control and experimental groups were blinded prior to scoring and all experiments were performed at least three times, with independent clutches. Scale bars in (D) and (E) represent 100 μm. |
Reprinted from Developmental Cell, 58(12), Hagedorn, E.J., Perlin, J.R., Freeman, R.J., Wattrus, S.J., Han, T., Mao, C., Kim, J.W., Fernández-Maestre, I., Daily, M.L., D'Amato, C., Fairchild, M.J., Riquelme, R., Li, B., Ragoonanan, D.A.V.E., Enkhbayar, K., Henault, E.L., Wang, H.G., Redfield, S.E., Collins, S.H., Lichtig, A., Yang, S., Zhou, Y., Kunar, B., Gomez-Salinero, J.M., Dinh, T.T., Pan, J., Holler, K., Feldman, H.A., Butcher, E.C., van Oudenaarden, A., Rafii, S., Junker, J.P., Zon, L.I., Transcription factor induction of vascular blood stem cell niches in vivo, 1037-1051.e4, Copyright (2023) with permission from Elsevier. Full text @ Dev. Cell