Figure 4.

Measures of pPaxillin distribution in GFP+ cells of injured pax7a:egfp larvae in the presence or absence of 50 µM Y-27632. pPaxillin density within GFP+ cells at the myoseptum (a) and within the myotome (b) was measured by defining puncta per µm2 within a GFP+ cell. pPaxillin puncta distribution was measured along the longest axis of the GFP+ cell (defined as the axis towards the injury, arrow) relative to the nucleus (blue ball) in the front, F, or back, B, of the cell (c). A ratio was calculated from the number of puncta at the front and back normalized to the total number of puncta per cell for untreated animals or those treated with Y-27632 (d). The position of the nucleus was measured along the axis and expressed as a % relative to the leading (anterior) or trailing (posterior) edge. In animals treated with Y-27632 the relative position of the nucleus was more posteriorly located in GFP+ cells. Significance of difference was determined by an unpaired Student's t-test (***p < 0.001, **p < 0.01, *p < 0.05, N.S. p > 0.05) with n = 3 animals per condition. Scale bar 10 µm (c).