Figure 2.

Adhesion molecule localization is polarized in muSCs migrating to injuries. Vinculin is detectable at the myoseptum (arrowheads, a) and is present as puncta on GFP+ cells (arrows, b) in injured (labelled by asterisk) pax7a:egfp larvae. The activated adhesion protein phosphoY118-Paxillin could be detected weakly at the myoseptum in pax7a:egfp larvae (c) and was present as puncta in GFP+ cells at the myoseptum (d) and within the myotome (e). Quantification of adhesion density within GFP+ cells was performed using Imaris with puncta defined as showing a diameter > 1.2 µm (f, n = 16 cells) and distribution of puncta was characterized by defining the ratio at the front compared to the back of a cell relative to the position of the nucleus (g, n = 16 cells). Statistical comparisons were performed by Student t-test and significance indicated (**p < 0.01, N.S. not significant) with n = 3 animals per condition. Scale bars 50 µm (a,c), 10 µm (b,d,e).