FIGURE

Figure 4.

ID

ZDB-FIG-220328-19

Publication

Krishnan et al., 2022 - Rab11 endosomes and Pericentrin coordinate centrosome movement during pre-abscission in vivo

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Figure 4.

Rab11 GTPase function is associated with centrosome bridge-directed movement.

(A) Control and Rab11-null expressing GFP-FIP3 (cyan, merge) human (HeLa) cells fixed and immunostained for Rab11 (inverted grays, left; magenta, merge). (B) Time-lapse of control and Rab11-null centrin-GFP (inverted grays) pre-abscising human (HeLa) cells. Pink arrows, centrosome. Dashed lines, cell boundaries (A, B). Scale bar, 10 μm Video 3. (C, D, E, F) Rab11-null cells expressing mCh-Rab11, -Rab11 (S25N), or -Rab11(Q70L). (C) Number of centrosomes per pre-abscising cell with bridge directed centrosome movement calculated as both centrosomes (2 centrosomes), only one centrosome (1 centrosome) and neither centrosome (0 centrosomes) moved shown as a violin plot with median (orange dashed line) and quartiles (dark dotted lines). One-way ANOVA, with Dunnett’s multiple comparison to control, ***P < 0.001, **P < 0.01 and n.s. not significant. (D) GFP-FIP3 Rab11-null cells expressing mCh-Rab11, -Rab11(S25N) or -Rab11(Q70L) (inverted grays, left; cyan, merge) fixed and immunostained for Pericentrin (magenta, merge). (E) Centrin-GFP (inverted grays) Rab11-null pre-abscising human (HeLa) cells expressing mCh-Rab11, -Rab11(S25N), or -Rab11(Q70L). Pink arrows, centrosome. Dashed lines, cell boundaries (D, E). Scale bar, 10 μm Video 4. (F) Directional displacement of centrosome towards cytokinetic bridge (scatter plot). Median (orange dashed line) and quartiles (dark lines) shown. One-way ANOVA with Dunnett’s multiple comparison to control, ***P < 0.001 and n.s. not significant. (C, F) n values, statistical results detailed in Table S1.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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