FIGURE

Figure 6.

ID
ZDB-FIG-220305-33
Publication
Ying et al., 2022 - The non-telomeric evolutionary trajectory of TRF2 in zebrafish reveals its specific roles in neurodevelopment and aging
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Figure 6.

zfTRF2 plays a specific role in GFAP-positive cells during neurodevelopment. (A) Percentages of embryos exhibiting brain edema phenotypes at 3 dpf in terfa-compromised embryos following microinjection with the plasmids described in (Supplementary Figure S6A). (B) Representative three-dimensional (3D) computationally-reconstructed brain images of living embryos. Images were taken via light-sheet microscopy. Green signals indicate neurons of terfa-compromised Tg(huc:GFP) embryos at 3 dpf after microinjection with the plasmid of expression zfTRF2 meditate by GFAP promotor. GFP-positive cells in whole brains were quantified using Imaris software (Bitplane). scale bars, 150 μm. (C) Representative confocal section images (right) and quantification (left) showing γH2AX foci colocalized with GFP-positive or mCherry-positive cells in the brains of terfa-compromised Tg(gfap:GFP) embryos at 3 dpf, following microinjection plasmid of expression zfTRF2 meditate by GFAP promotor. The quantification (left) shows that percentages of GFP-positive cells that colocalized with γH2AX foci in terfa-compromised Tg(gfap:GFP) embryos, scale bars, 30 μm. (D) Transcript levels of candidate genes from RNA-seq in terfa+/+, terfa+/− and terfa−/− embryos at 3 dpf. Embryos were microinjected with the plasmids described in Supplementary Figure S6A. The mRNA transcript levels were measured using RT-qPCR. All data are shown as the mean ± SEM of three biological replicates. The number below each column indicates the number of embryos detected for each condition. Statistical analysis was performed using the unpaired two-sided t test, and survival curves were plotted using the log-rank test (*P < 0.05). Asterisks directly above columns indicate a significant difference between the indicated treatment and the control group (left). Asterisks above two columns indicate a significant difference between the two columns.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nucleic Acids Res.