Figure 9

Figure 9. Evaluation of blood flow velocity and oxygen consumption in zebrafish larvae after co-incubating RAC and PROP. (A) Maximal and (B) average blood flow velocity in the dorsal aorta of zebrafish larvae aged at 72-h post fertilization (hpf) after 24 h incubations in RAC–PROP from 48 hpf onwards. The data are shown as the means ± SEM and were analyzed by One-way ANOVA test with Fisher’s LSD test (n Control = 41, n R4 = 40, n R4P1 = 20, and n R4P4 = 20). (C) Oxygen consumption of zebrafish larvae at 5 dpf after 24-h incubation in different doses of RAC and RAC-PROP combination. The data are shown as the means ± SD and analyzed by One-way ANOVA test with Dunn’s correction as post-hoc multiple comparison test (n = 42–46). Different letters a, b, and c above columns indicate significant statistical differences with p < 0.05. Abbreviations: R4 (RAC 4 ppm), R4P1 (RAC 4 ppm, PROP 1 ppm), R4P4 (RAC 4 ppm, PROP 4 ppm), R8 (RAC 8 ppm), R8P1 (RAC 8 ppm, PROP 1 ppm), and R8P4 (RAC 8 ppm, PROP 4 ppm). Black bar: control, orange bar: R4, silver bar: R4P1, gold bar: R4P4, blue bar: R8, cream bar: R8P1, and pink bar: R8P4.