FIGURE

Fig. S3

ID

ZDB-FIG-200805-23

Publication

Ye et al., 2020 - p53 isoform Δ113p53 promotes zebrafish heart regeneration by maintaining redox homeostasis

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Fig. S3

Validation of Tg(Δ113p53:CreER; β-act2:RSG) zebrafish. (A) Western blot with a monoclonal antibody against zebrafish Δ113p53 was performed to analyze the induction of Δ113p53 upon the treatment of a DNA damage drug, camptothecin (Campt). The Tg(Δ113p53:CreER; β-act2:RSG) zebrafish embryos at 1 day post fertilization (dpf) were treated with Campt for 24 hours. Afterwards, a part of untreated and Campt-treated embryos were divided and treated with 4HT for 2 hours. β-actin was used as the protein loading control. (B-E) Life images of red (DsRed) (B, C, D, E), green (EGFP) (B’, C’, D’, E’) and bright field (B”, C”, D”, E”) in Tg(Δ113p53:CreER; β-act2:RSG) zebrafish embryos treated with either Campt (C, C’, C”), or 4HT (D, D’, D”), or both (E, E’, E”) as described above. Scale bar, 500 μm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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