FIGURE

Fig. 4

ID

ZDB-FIG-200729-7

Publication

Ye et al., 2020 - p53 isoform Δ113p53 promotes zebrafish heart regeneration by maintaining redox homeostasis

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Fig. 4

The depletion of <italic>Δ113p53</italic> significantly decreases the proliferation frequency of cardiomyocytes during heart regeneration.

a, b The DsRed⁺ nucleus of cardiomyocytes (in red) at the resection site in Tg(myl7:nDsRed); Δ113p53^+/+(a) and Tg(myl7:nDsRed); Δ113p53^M/M (b) hearts were labelled by EdU (in green) at 14 dpa. Framed areas were magnified in a′ and b′. nDsRed: nuclear DsRed. The white arrow heads indicate co-labelling. Scale bar, 50 μm. c, d Cryosections of Tg(myl7:nDsRed); Δ113p53^+/+ (c) and Tg(myl7:nDsRed); Δ113p53^M/M (d) hearts at 7 dpa were co-stained by anti-DsRed and anti-PCNA antibodies. The nucleus were stained with DAPI (in blue). Framed areas were magnified in c′ and c″, or d′ and d″. The white arrow heads indicate co-labelling. Scale bar, 50 μm. e, f The number of co-labelled nDsRed⁺ nucleus of cardiomyocytes with either EdU (e) or PCNA (f) in Tg(myl7:nDsRed); Δ113p53^+/+ and Tg(myl7:nDsRed); Δ113p53^M/M hearts at 14 or 7 dpa, was presented as the percentage of the total nDsRed⁺ nucleus at the resection site. Data are means of three sections/heart. Each dot represents the average number of co-labelled nDsRed⁺ nucleus of cardiomyocytes with either EdU or PCNA in an individual heart. The experiments were repeated independently for at least three times with similar results. Statistical analysis was performed on relevant data using Student’s two-tailed t test in GraphPad Prism 5. The p values were represented by n.s. and asterisks. n.s., p > 0.05. *p < 0.05. **p < 0.01. ***p < 0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

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Acknowledgments

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