FIGURE

Figure 2-S1

ID

ZDB-FIG-200523-61

Publication

Kuil et al., 2020 - Zebrafish macrophage developmental arrest underlies depletion of microglia and reveals Csf1r-independent metaphocytes

Other Figures

All Figure Page

Back to All Figure Page

Figure 2-S1

<italic>csf1r<sup>DM</sup></italic> macrophages and microglia have a proliferation defect.

(A) Normalized CPM values of genes involved in DNA replication/cell cycle. (B) Representative images of L-plastin immunohistochemistry of microglia in different csf1r^DM larvae at 4 dpf in the midbrain. Scale bar represents 20 µM. Quantification of L-plastin+ microglia in control and different csf1r mutants at 2, 3 and 4 dpf and quantification Pcna+/L-plastin double positive microglia at 2, 3 and 4 dpf. (C) qPCR analysis on 10–15 whole embryos/larvae per group at different developmental timepoints, the number of replicates are depicted in Table 2. (D) qPCR analysis on different organs isolated from adult zebrafish (3–5 organs/sample), the number of replicates are depicted in Table 2. Statistical significance is calculated using one-way ANOVA with Dunnett’s multiple comparison test *<0,05 **<0,01 ***<0001. Each dot represents one fish.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Elife