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Figure 2-S1

ID: ZDB-IMAGE-200523-61
Publication: Kuil et al., 2020 - Zebrafish macrophage developmental arrest underlies depletion of microglia and reveals Csf1r-independent metaphocytes
All Figures: Figures for Kuil et al., 2020

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Figure 2-S1 <italic>csf1r<sup>DM</sup></italic> macrophages and microglia have a proliferation defect.

(A) Normalized CPM values of genes involved in DNA replication/cell cycle. (B) Representative images of L-plastin immunohistochemistry of microglia in different csf1r^DM larvae at 4 dpf in the midbrain. Scale bar represents 20 µM. Quantification of L-plastin+ microglia in control and different csf1r mutants at 2, 3 and 4 dpf and quantification Pcna+/L-plastin double positive microglia at 2, 3 and 4 dpf. (C) qPCR analysis on 10–15 whole embryos/larvae per group at different developmental timepoints, the number of replicates are depicted in Table 2. (D) qPCR analysis on different organs isolated from adult zebrafish (3–5 organs/sample), the number of replicates are depicted in Table 2. Statistical significance is calculated using one-way ANOVA with Dunnett’s multiple comparison test *<0,05 **<0,01 ***<0001. Each dot represents one fish.

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