ZFIN ID: ZDB-FIG-200521-12
Caetano-Lopes et al., 2020 - Unique and non-redundant function of csf1r paralogues in regulation and evolution of post-embryonic development of the zebrafish. Development (Cambridge, England)   147(2) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage Range: Days 45-89 to Adult

Fig. 2 Adult phenotypes of csf1ra and csf1rb mutants.

(A-D) Representative photomicrographs of adult (A) wild-type zebrafish (wt), (B) homozygous csf1ramh5, (C) csf1rbmh108 and (D) csf1ramh5;csf1rbmh112 mutant zebrafish. (E,F) Quantitative RT-PCR of csf1ra and csf1rb in isolated scales of wild type, or mutants singly homozygous for csf1ra (a−/−) or csf1rb (b−/−), and of double mutants (a−/−b−/−). (G,H) Representative photomicrographs of scales collected from 10 wpf wild-type fish stained using TRAP (G, arrow) and (H) modified Von Kossa staining (arrow). (I,J) Quantitation of TRAP and Von Kossa staining in wild-type and mutant scales. For TRAP activity, each data point represents the percentage of scales stained by TRAP obtained by evaluating 10 scales per fish. For Von Kossa analysis, each data point represents the percentage of resorbed area per individual scale. Data are mean±s.d. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
csf1ra AB standard conditions Adult scale RTPCR
csf1ramh5/mh5(AB) standard conditions Adult Not Detected scale RTPCR
csf1ramh5/mh5 ; csf1rbmh112/mh112(AB) standard conditions Adult Not Detected scale RTPCR
csf1rbmh108/mh108(AB) standard conditions Adult scale RTPCR
csf1rb AB standard conditions Adult scale RTPCR
csf1ramh5/mh5(AB) standard conditions Adult scale RTPCR
csf1ramh5/mh5 ; csf1rbmh112/mh112(AB) standard conditions Adult Not Detected scale RTPCR
csf1rbmh108/mh108(AB) standard conditions Adult Not Detected scale RTPCR
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
csf1ramh5/mh5(AB) standard conditions Days 45-89 scale bone resorption decreased occurrence, abnormal
Adult integument has fewer parts of type melanocyte, abnormal
Adult integument lacks all parts of type xanthophore, abnormal
Adult pigment cell development decreased process quality, abnormal
Adult scale csf1ra expression absent, abnormal
Adult scale csf1rb expression decreased amount, abnormal
csf1ramh5/mh5 ; csf1rbmh112/mh112(AB) standard conditions Days 45-89 scale bone resorption decreased occurrence, exacerbated
Adult integument has fewer parts of type melanocyte, abnormal
Adult integument lacks all parts of type xanthophore, abnormal
Adult pigment cell development decreased process quality, abnormal
Adult scale csf1ra expression absent, abnormal
Adult scale csf1rb expression absent, abnormal
csf1rbmh108/mh108(AB) standard conditions Days 45-89 scale bone resorption decreased occurrence, abnormal
Adult integument has normal numbers of parts of type melanocyte, normal
Adult integument has normal numbers of parts of type xanthophore, normal
Adult pigment cell development process quality, normal
Adult scale csf1rb expression absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development