ZFIN ID: ZDB-FIG-200521-13
Caetano-Lopes et al., 2020 - Unique and non-redundant function of csf1r paralogues in regulation and evolution of post-embryonic development of the zebrafish. Development (Cambridge, England)   147(2) Full text @ Development
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 3 Loss of csf1r paralogue function leads to unique and shared phenotypes in the skeleton.

MicroCT imaging and quantification of skeletal metrics of the spine of age-matched csf1r mutants and wild-type adult siblings. (A-D) MicroCT renderings of the lateral aspect of the spine showing overall morphology and integration of serial vertebrae. The neural spines (ns) and hemal spines (hs) are indicated. The measure used for the quantification of angle of the arch (J) is noted in A. (E-G,J,K) Quantification of vertebral shape and density in csf1r single and double mutants: (E) bone mineral density (BMD), (F) bone volume, (G) vertebral radius, (J) angle and (K) area of the neural arch. All measurements, with the exception of angle, were standardized to standard length (STL). (H,I) visualization of neural arch area in wild-type (H) and csf1ra; csf1rb mutant (I) zebrafish; measured area used for quantification of the arch area (K) is indicated in red in H. Data are meanĀ±s.d. *P<0.05, **P<0.01, ***P<0.001, ****P<0.0001.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
csf1ramh5/mh5 standard conditions Adult centrum increased diameter, abnormal
Adult centrum increased mass density, abnormal
Adult centrum normal volume, normal
Adult centrum bone mineralization increased process quality, abnormal
Adult neural arch dorsal orientation, abnormal
Adult neural arch normal area, normal
csf1ramh5/mh5 ; csf1rbmh112/mh112 standard conditions Adult centrum decreased volume, abnormal
Adult centrum increased diameter, abnormal
Adult centrum increased mass density, exacerbated
Adult centrum bone mineralization increased process quality, exacerbated
Adult neural arch decreased area, abnormal
Adult neural arch dorsal orientation, abnormal
csf1rbmh108/mh108 standard conditions Adult centrum decreased volume, abnormal
Adult centrum increased mass density, abnormal
Adult centrum normal diameter, normal
Adult centrum bone mineralization increased process quality, abnormal
Adult neural arch dorsal orientation, abnormal
Adult neural arch normal area, normal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development