csf1r genes show combinatorial effects in regulating patterning of the dentition and tooth number.
(A-D,F) Alizarin Red stained pharyngeal arches from 4 month post-fertilization (mpf) csf1r mutants and wild-type siblings. Numbers on the bottom represent the range of tooth numbers on each arch (n=7). Arrows indicate buildup of bone surrounding base of teeth in csf1ra and csf1rb mutants (B,C). (E) Quantification of the number of teeth represented in csf1r mutants, double mutants and wild-type siblings (****P<0.0001). (F) Higher magnification images of dentitions from csf1ra;csf1rb double mutants showing organization of supernumerary teeth into distinct tracts (yellow line) associated with a row of normal tooth germ patterning in wild-type dentitions (Fig. S6). (G-J) Localization of ctsk-positive cells (magenta) on the dentary of zebrafish csf1r mutants. Bottom, overlay with calcein stain (cyan).