ZFIN ID: ZDB-FIG-200521-14
Caetano-Lopes et al., 2020 - Unique and non-redundant function of csf1r paralogues in regulation and evolution of post-embryonic development of the zebrafish. Development (Cambridge, England)   147(2) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Term:
Stage: Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Adult

Fig. 4 csf1r genes show combinatorial effects in regulating patterning of the dentition and tooth number.

(A-D,F) Alizarin Red stained pharyngeal arches from 4 month post-fertilization (mpf) csf1r mutants and wild-type siblings. Numbers on the bottom represent the range of tooth numbers on each arch (n=7). Arrows indicate buildup of bone surrounding base of teeth in csf1ra and csf1rb mutants (B,C). (E) Quantification of the number of teeth represented in csf1r mutants, double mutants and wild-type siblings (****P<0.0001). (F) Higher magnification images of dentitions from csf1ra;csf1rb double mutants showing organization of supernumerary teeth into distinct tracts (yellow line) associated with a row of normal tooth germ patterning in wild-type dentitions (Fig. S6). (G-J) Localization of ctsk-positive cells (magenta) on the dentary of zebrafish csf1r mutants. Bottom, overlay with calcein stain (cyan).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
DsRed csf1ramh5/mh5 ; mh201Tg standard conditions Adult ceratobranchial 5 tooth osteoclast IFL
csf1rbmh108/mh108 ; mh201Tg standard conditions Adult ceratobranchial 5 tooth osteoclast IFL
csf1rbmh112/mh112 ; csf1ramh5/mh5 ; mh201Tg standard conditions Adult ceratobranchial 5 tooth osteoclast IFL
mh201Tg standard conditions Adult ceratobranchial 5 tooth osteoclast IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
csf1ramh5/mh5 standard conditions Adult ceratobranchial 5 bone has normal numbers of parts of type ceratobranchial 5 tooth, normal
Adult ceratobranchial 5 tooth basal surface osseous, abnormal
csf1ramh5/mh5 ; csf1rbmh112/mh112 standard conditions Adult ceratobranchial 5 bone has extra parts of type ceratobranchial 5 tooth, abnormal
Adult ceratobranchial 5 bone odontogenesis increased occurrence, abnormal
csf1rbmh108/mh108 standard conditions Adult ceratobranchial 5 bone has normal numbers of parts of type ceratobranchial 5 tooth, normal
Adult ceratobranchial 5 tooth basal surface osseous, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development