FIGURE

Fig. 7

ID

ZDB-FIG-200212-8

Publication

Song et al., 2020 - Tetrahydrobenzimidazole TMQ0153 triggers apoptosis, autophagy and necroptosis crosstalk in chronic myeloid leukemia

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Fig. 7

TMQ0153 induced mitochondrial dysfunction and MMP in K562 cells.

a Cells were treated with TMQ0153 for 8 h and mitochondrial morphology was assessed by TEM at 12.000x and 25.000x magnification. Single arrows and double arrows indicate respectively dilated and giant mitochondria, asterisks indicate autophagophores. b Mitochondrial membrane potential (MMP) analysis in cells treated with increasing concentrations of TMQ0153 for 24 h. The fraction of low MMP presenting cells is depicted. c Cells were pre-incubated for 1 h in presence or absence of 50 mM N-acetyl-L-cysteine (NAC) followed by a treatment with the indicated concentrations of TMQ0153. After 24 h of treatment, MMP (upper panel) cell viability (bottom panel) were assessed by trypan blue assay and flow cytometry, respectively. All pictures are representative of three independent experiments and data represent the mean (±S.D.) of three independent experiments. Statistical significance was assessed as *p < 0.05, **p < 0.01, ***p < 0.001 compared to untreated cells unless otherwise specified. One-way ANOVA (mitochondrial membrane); post hoc; Dunnett’s test. One-way ANOVA (cell viability); post hoc; Tukey’s test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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