ZFIN ID: ZDB-FIG-190620-13
Clément et al., 2018 - Cog4 is required for protrusion and extension of the epithelium in the developing semicircular canals. Mechanisms of Development   155:1-7 Full text @ Mech. Dev.
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-5 to Protruding-mouth

Fig. 3

Formation of the epithelial projections is delayed in cog4−/−mutants. Live images of the inner ear from cog4+/+ sibling (A, n = 49 embryos; C, n = 57 larvae; E, n = 26 larvae) and cog4−/− mutant larvae (B, n = 21 embryos; D, n = 22 larvae; F, n = 18 larvae) at 28 hpf (A, B), 52 hpf (C, D) and 75 hpf (E, F). Orange stars indicate the anterior and posterior projections and bulges (C, D). The red and blue arrowheads indicate the anterior and forming posterior pillar, respectively (C). Black arrowheads point to the gaps between the anterior and forming posterior projections and bulges (D, F). Anterior to the left (A–F). Dorsal to the top (A, B, E, F). Medial to the top (C, D). Percentage of inner ears with projections growing correctly at 52 hpf and 75 hpf, in cog4+/+ sibling (52 hpf, n = 114 inner ears; 75 hpf, n = 26 inner ears) and cog4−/− mutant larvae (52 hpf, n = 38 inner ears; 75 hpf, n = 18 inner ears) (G). Ear size at 28, 52 and 75 hpf in cog4+/+ sibling (28 hpf, n = 49 embryos; 52 hpf, n = 18 larvae; 75 hpf, n = 26 larvae) and cog4−/− mutant larvae (28 hpf, n = 21 embryos; 52 hpf, n = 6 larvae; 75 hpf, n = 18 larvae) (H). Size was measured along the antero-posterior axis of the inner ear. Data are represented as mean ± SEM. **p < 0.01.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
cog4b1312/b1312 standard conditions Prim-5 otic vesicle morphology, normal
Long-pec otic vesicle lacks parts or has fewer parts of type otic vesicle protrusion, abnormal
Long-pec semicircular canal formation delayed, abnormal
Protruding-mouth inner ear lacks parts or has fewer parts of type semicircular canal, abnormal
Protruding-mouth pillar of the semicircular canal malformed, abnormal
Protruding-mouth semicircular canal formation delayed, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Mechanisms of Development for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Mechanisms of Development, 155, Clément, A., Blanco-Sánchez, B., Peirce, J.L., Westerfield, M., Cog4 is required for protrusion and extension of the epithelium in the developing semicircular canals, 1-7, Copyright (2018) with permission from Elsevier. Full text @ Mech. Dev.