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ZFIN ID: ZDB-FIG-190329-7
Montague et al., 2018 - Conserved regulation of Nodal-mediated left-right patterning in zebrafish and mouse. Development (Cambridge, England)   145(24) Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 10-13 somites to 20-25 somites
PHENOTYPE:
Fish:
Observed In:
Stage Range: 10-13 somites to Long-pec

Fig. 1

Spaw, Dand5 and Lefty1 are required for left-right asymmetry. (A) Expression patterns of spaw, vg1/gdf3, dand5 and lefty1 in wild-type zebrafish embryos. LPM, lateral plate mesoderm; N, notochord; KV, Kupffer's vesicle. (B) Spaw induces itself and its inhibitor Lefty1. Spaw is also inhibited by Dand5. (C) Wild-type (WT), dand5, lefty1 (lft1), spaw, dand5;lft1, dand5;spaw and lft1;spaw embryos at 28 h post-fertilization (hpf). See Table S1 for information about the mutant alleles. (D) Quantification of heart jogging at 28 hpf in wild-type and mutant embryos. (E) Quantification of heart looping at 2 days post-fertilization (dpf) in wild-type and mutant embryos, detected by expression of myl7/cmlc2 by in situ hybridization. D-loop, dextral loop; S-loop, sinistral loop. A large proportion of hearts failed to loop correctly in either direction, forming no loop or a very mild loop. We designated these embryos as ‘mild/no loop’. (F) Expression of lefty1, spaw and lefty2 (lft2) at the 10-somite stage (10SS) and 21SS in wild-type and mutant embryos. Each image is representative of three to five independent experiments (embryo number is in bottom right corner of each panel). Scale bar: 150 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
lft1 AB/TL standard conditions 10-13 somites notochord ISH
20-25 somites whole organism medial region ISH
dand5a204/a204(AB/TL) standard conditions 10-13 somites notochord ISH
20-25 somites whole organism medial region ISH
dand5a204/a204 ; spawa205/a205(AB/TL) standard conditions 10-13 somites Not Detected notochord ISH
20-25 somites Not Detected whole organism medial region ISH
lft1a145/a145(AB/TL) standard conditions 10-13 somites notochord ISH
20-25 somites whole organism medial region ISH
lft1a145/a145 ; dand5a204/a204(AB/TL) standard conditions 10-13 somites notochord ISH
20-25 somites whole organism medial region ISH
lft1a145/a145 ; spawa205/a205(AB/TL) standard conditions 10-13 somites Not Detected notochord ISH
20-25 somites Not Detected whole organism medial region ISH
spawa205/a205(AB/TL) standard conditions 10-13 somites Not Detected notochord ISH
20-25 somites Not Detected whole organism medial region ISH
lft2 AB/TL standard conditions 20-25 somites heart primordium left side ISH
dand5a204/a204(AB/TL) standard conditions 20-25 somites heart primordium left side ISH
20-25 somites heart primordium right side ISH
dand5a204/a204 ; spawa205/a205(AB/TL) standard conditions 20-25 somites Not Detected heart primordium ISH
lft1a145/a145(AB/TL) standard conditions 20-25 somites heart primordium left side ISH
20-25 somites heart primordium right side ISH
lft1a145/a145 ; dand5a204/a204(AB/TL) standard conditions 20-25 somites heart primordium left side ISH
20-25 somites heart primordium right side ISH
lft1a145/a145 ; spawa205/a205(AB/TL) standard conditions 20-25 somites Not Detected heart primordium ISH
spawa205/a205(AB/TL) standard conditions 20-25 somites Not Detected heart primordium ISH
spaw AB/TL standard conditions 10-13 somites Kupffer's vesicle anatomical region ISH
dand5a204/a204(AB/TL) standard conditions 10-13 somites Kupffer's vesicle anatomical region ISH
10-13 somites lateral plate mesoderm left side ISH
10-13 somites lateral plate mesoderm right side ISH
dand5a204/a204 ; spawa205/a205(AB/TL) standard conditions 10-13 somites Kupffer's vesicle anatomical region ISH
lft1a145/a145(AB/TL) standard conditions 10-13 somites lateral plate mesoderm left side ISH
10-13 somites lateral plate mesoderm right side ISH
10-13 somites notochord ISH
lft1a145/a145 ; dand5a204/a204(AB/TL) standard conditions 10-13 somites lateral plate mesoderm left side ISH
10-13 somites lateral plate mesoderm right side ISH
10-13 somites notochord ISH
lft1a145/a145 ; spawa205/a205(AB/TL) standard conditions 10-13 somites Kupffer's vesicle anatomical region ISH
spawa205/a205(AB/TL) standard conditions 10-13 somites Kupffer's vesicle anatomical region ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
dand5a204/a204(AB/TL) standard conditions 10-13 somites lateral plate mesoderm left side spaw expression mislocalised, abnormal
10-13 somites lateral plate mesoderm right side spaw expression mislocalised, abnormal
10-13 somites notochord lft1 expression increased amount, abnormal
10-13 somites notochord lft1 expression increased distribution, abnormal
20-25 somites heart primordium right side lft2 expression mislocalised, abnormal
20-25 somites whole organism medial region lft1 expression increased amount, abnormal
20-25 somites whole organism medial region lft1 expression increased distribution, abnormal
Prim-5 heart jogging decreased occurrence, abnormal
Prim-5 heart jogging process quality, abnormal
Long-pec heart looping decreased occurrence, abnormal
Long-pec heart looping process quality, abnormal
dand5a204/a204 ; spawa205/a205(AB/TL) standard conditions 10-13 somites Kupffer's vesicle anatomical region spaw expression decreased amount, abnormal
10-13 somites notochord lft1 expression absent, abnormal
20-25 somites heart primordium lft2 expression absent, abnormal
20-25 somites whole organism medial region lft1 expression absent, abnormal
Prim-5 heart jogging decreased occurrence, abnormal
Prim-5 heart jogging process quality, abnormal
Long-pec heart looping decreased occurrence, abnormal
Long-pec heart looping process quality, abnormal
lft1a145/a145(AB/TL) standard conditions 10-13 somites lateral plate mesoderm left side spaw expression mislocalised, abnormal
10-13 somites lateral plate mesoderm right side spaw expression mislocalised, abnormal
10-13 somites notochord lft1 expression increased amount, abnormal
10-13 somites notochord lft1 expression increased distribution, abnormal
10-13 somites notochord spaw expression mislocalised, abnormal
20-25 somites heart primordium right side lft2 expression mislocalised, abnormal
20-25 somites whole organism medial region lft1 expression increased amount, abnormal
20-25 somites whole organism medial region lft1 expression increased distribution, abnormal
Prim-5 heart jogging decreased occurrence, abnormal
Prim-5 heart jogging process quality, abnormal
Long-pec heart looping decreased occurrence, abnormal
Long-pec heart looping process quality, abnormal
lft1a145/a145 ; dand5a204/a204(AB/TL) standard conditions 10-13 somites lateral plate mesoderm left side spaw expression mislocalised, abnormal
10-13 somites lateral plate mesoderm right side spaw expression mislocalised, abnormal
10-13 somites notochord lft1 expression increased amount, abnormal
10-13 somites notochord lft1 expression increased distribution, abnormal
10-13 somites notochord spaw expression mislocalised, abnormal
20-25 somites heart primordium right side lft2 expression mislocalised, abnormal
20-25 somites whole organism medial region lft1 expression increased amount, abnormal
20-25 somites whole organism medial region lft1 expression increased distribution, abnormal
Prim-5 heart jogging decreased occurrence, abnormal
Prim-5 heart jogging process quality, abnormal
Long-pec heart looping decreased occurrence, abnormal
Long-pec heart looping process quality, abnormal
lft1a145/a145 ; spawa205/a205(AB/TL) standard conditions 10-13 somites Kupffer's vesicle anatomical region spaw expression decreased amount, abnormal
10-13 somites notochord lft1 expression absent, abnormal
20-25 somites heart primordium lft2 expression absent, abnormal
20-25 somites whole organism medial region lft1 expression absent, abnormal
Prim-5 heart jogging decreased occurrence, abnormal
Prim-5 heart jogging process quality, abnormal
Long-pec heart looping decreased occurrence, abnormal
Long-pec heart looping process quality, abnormal
spawa205/a205(AB/TL) standard conditions 10-13 somites Kupffer's vesicle anatomical region spaw expression decreased amount, abnormal
10-13 somites notochord lft1 expression absent, abnormal
20-25 somites heart primordium lft2 expression absent, abnormal
20-25 somites whole organism medial region lft1 expression absent, abnormal
Prim-5 heart jogging decreased occurrence, abnormal
Prim-5 heart jogging process quality, abnormal
Long-pec heart looping decreased occurrence, abnormal
Long-pec heart looping process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development