Spaw, Dand5 and Lefty1 are required for left-right asymmetry. (A) Expression patterns of spaw, vg1/gdf3, dand5 and lefty1 in wild-type zebrafish embryos. LPM, lateral plate mesoderm; N, notochord; KV, Kupffer's vesicle. (B) Spaw induces itself and its inhibitor Lefty1. Spaw is also inhibited by Dand5. (C) Wild-type (WT), dand5, lefty1 (lft1), spaw, dand5;lft1, dand5;spaw and lft1;spaw embryos at 28 h post-fertilization (hpf). See Table S1 for information about the mutant alleles. (D) Quantification of heart jogging at 28 hpf in wild-type and mutant embryos. (E) Quantification of heart looping at 2 days post-fertilization (dpf) in wild-type and mutant embryos, detected by expression of myl7/cmlc2 by in situ hybridization. D-loop, dextral loop; S-loop, sinistral loop. A large proportion of hearts failed to loop correctly in either direction, forming no loop or a very mild loop. We designated these embryos as ‘mild/no loop’. (F) Expression of lefty1, spaw and lefty2 (lft2) at the 10-somite stage (10SS) and 21SS in wild-type and mutant embryos. Each image is representative of three to five independent experiments (embryo number is in bottom right corner of each panel). Scale bar: 150 µm.

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.