ZFIN ID: ZDB-FIG-180727-13
Guerra et al., 2018 - Distinct myocardial lineages break atrial symmetry during cardiogenesis in zebrafish. eLIFE   7 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 5

Atrial laterality is established from distinct antero-posterior fields without contribution from the second heart field.

(a, c) Cardiac disc (dotted circle) at 23 ss. (b–b’) Confocal projections of photoconverted kikGR with Tg(myl7:BFP-CAAX) hearts (yellow lines) show that cells from the PDC populate the left side of the atrium while (d–d’) cells from the ADC populate the right side of the atrium (white dotted lines). (b’’, d’’) Single optical planes from b’ and d’, respectively (cardiomyocytes, green arrows; endocardial cells, white arrows). (e) Tg(meis2b-reporter) expression and antibody staining for Isl1 in the 48 hpf heart [meis2b+/Isl1+ (white arrows), meis2b+/Isl1- (green arrows) and meis2b-/Isl1+ (pink arrows) cardiomyocytes]. (f) Confocal projections of control uninjected and isl1 MO injected 48 hpf Tg(meis2b-reporter);Tg(myl7:mCherry) hearts [white lines delineate Tg(meis2b-reporter) expression]. Scale bars: 100 µm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP bns15Tg ; nkuasgfp1aTg standard conditions Long-pec atrial myocardium left side IHC
Long-pec Not Detected atrial myocardium right side IHC
Long-pec atrium cardiac muscle cell IHC
bns15Tg; chb1Tg; nkuasgfp1aTg standard conditions Long-pec atrial myocardium left side IFL
Long-pec Not Detected atrial myocardium right side IFL
bns15Tg; chb1Tg; nkuasgfp1aTg + MO4-isl1a standard conditions Long-pec atrial myocardium left side IFL
Long-pec Not Detected atrial myocardium right side IFL
isl1a bns15Tg ; nkuasgfp1aTg standard conditions Long-pec atrium cardiac muscle cell IHC
mCherry bns15Tg; chb1Tg; nkuasgfp1aTg standard conditions Long-pec atrial endocardium IFL
Long-pec ventricular myocardium IFL
bns15Tg; chb1Tg; nkuasgfp1aTg + MO4-isl1a standard conditions Long-pec atrial endocardium IFL
Long-pec ventricular myocardium IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
bns15Tg; chb1Tg; nkuasgfp1aTg + MO4-isl1a standard conditions Long-pec atrium determination of heart left/right asymmetry process quality, normal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife