ZFIN ID: ZDB-FIG-180727-15
Guerra et al., 2018 - Distinct myocardial lineages break atrial symmetry during cardiogenesis in zebrafish. eLIFE   7 Full text @ Elife
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Anatomical Terms:
Stage: Days 30-44
PHENOTYPE:
Fish:
Observed In:
Stage Range: Days 21-29 to Adult

Fig. 6

Loss of meis2b causes dysmorphic atrial growth and conduction defects.

(a) meis2b-/- larvae exhibit a significant increase in the ratio of atrial to ventricular surface area, while (b) ventricular surface area and (c) body length are not affected compared to WT siblings. (d) Analysis of Tg(myl7:LIFEACT-GFP) fish reveals no obvious defects in atrial myofibrillar architecture in 4 wpf meis2b-/-. (e–h) Adult meis2b-/- display pericardial bulging (arrow) due to abnormal atrial growth. (i) meis2b-/- exhibit a significant increase in atrial cardiomyocyte (CM) proliferation at 3 mpf. (j–k) Cardiac physiology assessed using electrocardiography (ECG) at 5 and 12 mpf. (j) meis2b-/- exhibit a significant increase in PR-segment variability compared to WT siblings, while P-duration and ventricular conduction (QRS) appear unaffected at 5 mpf. (k) At 12 mpf, PR-segment, P-duration and QRS interval are significantly affected in meis2b-/-. (l–m) Representative ECGs of 12 mpf animals show reduced PR-segment duration (red line) and multiple P-waves (arrow) in meis2b-/-. Scale bars: (d) 100 µm, (e–h) 1 mm. (a–c, i) One-tailed student t-tests were performed. (j–k) F-test and Bartlett’s test were performed. Significant differences compared with control are indicated (*p<0.05; **p<0.005; ***p<0.0005); error bars indicate ± SD. A, atrium; V, ventricle; ns, not significant.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
GFP meis2bs988/s988; s974Tg standard conditions Days 30-44 atrial myocardium cardiac myofibril IFL
Days 30-44 ventricular myocardium cardiac myofibril IFL
s974Tg standard conditions Days 30-44 atrial myocardium cardiac myofibril IFL
Days 30-44 ventricular myocardium cardiac myofibril IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
meis2bs988/s988 standard conditions Days 21-29 atrium increased area, abnormal
Days 21-29 cardiac atrium morphogenesis decreased process quality, abnormal
Days 21-29 cardiac ventricle area, normal
Days 21-29 whole organism length, normal
Days 30-44 atrium cardiac myofibril morphology, normal
Days 30-44 atrium myofibril assembly process quality, normal
Adult atrial myocardium cell population proliferation increased occurrence, abnormal
Adult atrium cardiac conduction decreased process quality, abnormal
Adult atrium increased area, abnormal
Adult cardiac atrium morphogenesis decreased process quality, abnormal
meis2bs988/s988; s974Tg standard conditions Days 30-44 atrium increased size, abnormal
Days 30-44 cardiac atrium morphogenesis decreased process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal eLIFE for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Elife