ZFIN ID: ZDB-FIG-180427-9
Miles et al., 2017 - Mis-expression of grainyhead-like transcription factors in zebrafish leads to defects in enveloping layer (EVL) integrity, cellular morphogenesis and axial extension. Scientific Reports   7:17607 Full text @ Sci. Rep.
Anatomical Terms:
Stage Range: Bud to Prim-5

Fig. S2

Expression patterns of grhl3 and arhgef19 in early zebrafish development.

(A-F) grhl3 is expressed within the pharyngeal endoderm (PE) from 10 hpf (A), a timepoint at which expression within the EVL is also visible. Expression within both these regions persists until at least 18 hpf (B-C). grhl3 expression is not seen in brain; non-specific probe trapping (C) is confirmed as artefact following both brightfield imaging of coronal sections (D) and subsequent H&E staining of both coronal (E) and sagittal (F) sections. Note persistent expression of grhl3 within the PE (D-F) and occasional puncta of staining within the EVL (F). (G-H) Expression of arhgef19 is seen in the EVL, lateral to the MHB (arrows, G), as well as in the EVL at the posterior-most region of the extending tail (arrow, H) at 24 hpf. (I) Q-RT-PCR showing expected non-significant decrease in arhgef19 and spec1 transcripts in MO:grhl3 injected embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
arhgef19 TU standard conditions Prim-5 post-vent region integument ISH
grhl3 TU standard conditions Bud endoderm anterior region ISH
Bud integument ISH
10-13 somites integument ISH
10-13 somites pharyngeal endoderm ISH
14-19 somites integument ISH
14-19 somites pharyngeal endoderm ISH
Prim-5 integument ISH
Prim-5 pharyngeal endoderm ISH
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.