ZFIN ID: ZDB-FIG-180427-9
Miles et al., 2017 - Mis-expression of grainyhead-like transcription factors in zebrafish leads to defects in enveloping layer (EVL) integrity, cellular morphogenesis and axial extension. Scientific Reports   7:17607 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Bud to Prim-5

Fig. S2

Expression patterns of grhl3 and arhgef19 in early zebrafish development.

(A-F) grhl3 is expressed within the pharyngeal endoderm (PE) from 10 hpf (A), a timepoint at which expression within the EVL is also visible. Expression within both these regions persists until at least 18 hpf (B-C). grhl3 expression is not seen in brain; non-specific probe trapping (C) is confirmed as artefact following both brightfield imaging of coronal sections (D) and subsequent H&E staining of both coronal (E) and sagittal (F) sections. Note persistent expression of grhl3 within the PE (D-F) and occasional puncta of staining within the EVL (F). (G-H) Expression of arhgef19 is seen in the EVL, lateral to the MHB (arrows, G), as well as in the EVL at the posterior-most region of the extending tail (arrow, H) at 24 hpf. (I) Q-RT-PCR showing expected non-significant decrease in arhgef19 and spec1 transcripts in MO:grhl3 injected embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
arhgef19 TU standard conditions Prim-5 post-vent region integument ISH
grhl3 TU standard conditions Bud endoderm anterior region ISH
Bud integument ISH
10-13 somites integument ISH
10-13 somites pharyngeal endoderm ISH
14-19 somites integument ISH
14-19 somites pharyngeal endoderm ISH
Prim-5 integument ISH
Prim-5 pharyngeal endoderm ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.