FIGURE

Fig. 4

ID

ZDB-FIG-180427-6

Publication

Miles et al., 2017 - Mis-expression of grainyhead-like transcription factors in zebrafish leads to defects in enveloping layer (EVL) integrity, cellular morphogenesis and axial extension

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Fig. 4

grhl2b/grhl3 double morphants present with phenotypes highly consistent with disrupted convergence-extension mediated migration, as well as MHB folding defects. (a–i) MO:grhl2b + MO:grhl3 injected double morphants (a) display a reduced axial length and decreased length:width ratio (LWR) relative to embryos injected with MO:control (b). These differences were quantitated (c), showing significantly decreased LWR in MO:grhl2b + MO:grhl3 injected double morphants (p < 0.0001 by Student’s T-test). (d–g) Dorsal views of MO:grhl2b + MO:grhl3 double morphants (d,f) relative to MO:control embryos (e,g) at 24 hpf highlighting the thicker trunk, broadened notochord (n) and splayed somites (s) characteristic of embryos with disrupted convergence-extension; as well as disruption of MHB morphogenesis (compare f with g). (h–i) MO:grhl2b + MO:grhl3 double morphants (f) relative to MO:control embryos at 96 hpf, highlighting severely reduced axial extension.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	TU + MO2-grhl2b + MO2-grhl3
Knockdown Reagents:	MO2-grhl2b MO2-grhl3
Observed In:	convergent extension involved in axis elongation midbrain hindbrain boundary neural tube midbrain-hindbrain boundary morphogenesis notochord post-vent region somite whole organism anterior-posterior axis
Stage Range:	Prim-5 to Day 4

Phenotype Detail

Acknowledgments

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