ZFIN ID: ZDB-FIG-180427-5
Miles et al., 2017 - Mis-expression of grainyhead-like transcription factors in zebrafish leads to defects in enveloping layer (EVL) integrity, cellular morphogenesis and axial extension. Scientific Reports   7:17607 Full text @ Sci. Rep.
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 3

EVL cell size and identity is aberrant in MO:grhl3 and MO:arhgef19 injected zebrafish. (ag) Rhodamine-phalloidin staining of F-actin in 24 hpf embryos injected with either MO:grhl3 + MO:arhgef19 MO:control (a,b) or MO:control (d,e) shows that periderm cells with abrogated grhl3/arhgef19 signalling are larger (boxed region in b,e). Morphometric quantitation of cell size using FiJi software (c,f) shows significant disparity in cell size in MO:grhl3 + MO: arhgef19 injected embryos (as well as singly-injected MO:grhl3 and MO: arhgef19 embryos), relative to controls (G). (ho) The basal keratinocyte marker fibronectin (i) is ectopically expressed in EVL cells surrounding the MHB in MO:grhl3-injected embryos (merged image; j). Conversely, EVL cells (k) do not express fibronectin (l) in MO:control fish (merged image, m). Digital cross-section confirms that several dorso-ventral layers of fibronectin positive cells are present in MO:grhl3-injected fish (n), whereas fibronectin positive cells are distributed within a single, one-cell thick layer MO:control injected fish (o).

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
TU + MO1-arhgef19 standard conditions Prim-5 periderm cell increased size, abnormal
TU + MO1-arhgef19 + MO2-grhl3 standard conditions Prim-5 periderm cell increased size, exacerbated
TU + MO2-grhl3 standard conditions Prim-5 periderm cell increased size, abnormal
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.