ZFIN ID: ZDB-FIG-180103-48
Iribarne et al., 2017 - Aipl1 is required for cone photoreceptor function and survival through the stability of Pde6c and Gc3 in zebrafish. Scientific Reports   7:45962 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage: Days 7-13

Fig. 5

zGc3 expression is coupled to Aipl1 and Pde6c in zebrafish photoreceptors.

(a) Labeling of wild-type, gosh, and els mutant retinas and pineal eyes at 7 dpf with anti-formaldehyde-fixed cGMP antibody (green) and zpr1 antibody (magenta). Control wild-type retinas show an undetectable level of cGMP. gosh and els mutant retinas show similar results, except that a few photoreceptors near the CMZ show very high accumulation of cGMP (arrow). On the other hand, pineal photoreceptors are positive for cGMP in gosh and els mutants, but not in wild type fish. (b) Western blot of wild-type, gosh and els mutant heads with anti-zGc3 antibody. A band of ~120 kDa is detected in wild-type heads, but disappears in both gosh and els mutant heads. (c) Semi-quantitative PCR of gc3 mRNA expression in wild-type, and gosh or els mutant embryos. mRNA levels are similar between wild-type, and gosh or els mutant embryos.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gucy2d WT standard conditions Days 7-13 retina photoreceptor cell RTPCR
aipl2oki6/oki6 standard conditions Days 7-13 retina photoreceptor cell RTPCR
pde6crw76a/rw76a standard conditions Days 7-13 retina photoreceptor cell RTPCR
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zpr-1 IHC aipl2oki6/oki6 standard conditions Days 7-13 retina
IHC pde6crw76a/rw76a standard conditions Days 7-13 retina
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.