ZFIN ID: ZDB-FIG-180103-46
Iribarne et al., 2017 - Aipl1 is required for cone photoreceptor function and survival through the stability of Pde6c and Gc3 in zebrafish. Scientific Reports   7:45962 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Protruding-mouth to Adult
PHENOTYPE:
Fish:
Observed In:
Stage: Protruding-mouth

Fig. 3

The gosh mutant gene encodes aipl1b.

(a) Schematic representation of human AIPL1 and zebrafish Aipl1b. FKBP and TPR domains are conserved. However, the zebrafish C-terminal region is not homologous to the PRD domain. In the gosh mutant, a nonsense mutation occurs in Gln413. (b) Phylogenetic tree of AIPL1 proteins of humans (Genbank no: AAF74023.1), mice (Genbank no: AAK77956.1), chicks (Genbank no: XP_001233085.3), Xenopus (Genbank no: XP_002933555.1), and zebrafish Aipl1a and Aipl1b. (c) Expression of aipl1a and aipl1b mRNAs in wild type at 72 hpf. aipl1b mRNA is expressed in the retina and the pineal eye (arrow). aipl1a mRNA is expressed in the brain, including the retina. Strong expression of aipl1a mRNA in the ventral patch of the retina. (d) Expression of aipl1a and aipl1b mRNAs in wild-type adult retina. aipl1b mRNA is expressed exclusively in cones, while aipl1a mRNA is expressed in rods and probably in short single (UV) cones (red arrowheads). ss, short single cone (red arrowheads); ls, long single cones (red outlined arrowhead); d, double cones (gray outlined arrowhead). (e) Expression of aipl1a and aipl1b mRNAs in wild-type and gosh mutant retinas at 72 hpf. aipl1b mRNA is markedly decreased in gosh mutant retinas, while aipl1a mRNA expression in ventral photoreceptors (arrows) is similar between wild-type and the gosh mutant retina.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
aipl1 WT standard conditions Protruding-mouth brain ISH
Protruding-mouth photoreceptor cell ventral side ISH
Protruding-mouth retina ISH
Adult retinal rod cell ISH
aipl2oki6/oki6 standard conditions Protruding-mouth photoreceptor cell ventral side ISH
aipl2 WT standard conditions Protruding-mouth pineal complex ISH
Protruding-mouth retina ISH
Adult retinal cone cell ISH
aipl2oki6/oki6 standard conditions Protruding-mouth retina ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
aipl2oki6/oki6 standard conditions Protruding-mouth retina aipl2 expression decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.