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ZIRC
ZFIN ID: ZDB-FIG-180103-47
Iribarne et al., 2017 - Aipl1 is required for cone photoreceptor function and survival through the stability of Pde6c and Gc3 in zebrafish. Scientific Reports   7:45962 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Anatomical Term:
Stage: Days 7-13
PHENOTYPE:
Fish:
Observed In:
Stage: Days 7-13

Fig. 4

The gosh mutant genetically interacts with the els mutant.

(a) Western blot of wild-type and gosh mutant heads with anti-Pde6c antibody. A band of approximately 100 kDa was detected in wild type animals, but disappeared in the gosh mutant. Histone H3 is as a loading control. (b) Semi-quantitative PCR with different numbers of cycles (20, 30 or 40) indicates that mRNA levels are similar between wild-type zebrafish and the gosh mutant. (c) Labeling of els+/+;gosh+/+, els−/−;gosh+/+, and els−/−;gosh+/− retinas with the zpr1 antibody. The ratio of zpr1-postive area relative to the total retinal area is significantly decreased in els−/−;gosh+/− retinas compared to els−/−;gosh+/+ retinas. ANOVA was significantly different (p< 0.000016) by t-test with a Bonferroni correction post-hoc (*p < 0.05; **p < 0.01; ***p < 0.001).

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zpr-1 IHC WT standard conditions Days 7-13 retina
IHC aipl2oki6/+ ; pde6crw76a/rw76a standard conditions Days 7-13 retina
IHC pde6crw76a/rw76a standard conditions Days 7-13 retina
Phenotype Details
Fish Conditions Stage Phenotype
aipl2oki6/+ ; pde6crw76a/rw76a standard conditions Days 7-13 retina photoreceptor cell decreased amount, abnormal
pde6crw76a/rw76a standard conditions Days 7-13 retina photoreceptor cell decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.