header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-171227-39
Pimtong et al., 2014 - Drl.3 governs primitive hematopoiesis in zebrafish. Scientific Reports   4:5791 Full text @ Sci. Rep.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: 2-cell to Day 5

Fig. 1

Drl gene family members are expressed throughout embryonic development and in multiple hematopoietic lineages.

(a) RT-PCR analysis of the drl family members and β−actin from pooled embryos at the indicated ages. (b) Semi-quantitative (q) RT-PCR of drl genes and β-actin from pooled 14 somite stage embryos (left panel). Arrows indicate the bands that were used for quantitation, which is shown in the right panel. Compared to drl, *P = 0.0150, **P = 0.0052,, xP = 0.0360,, *P = 0.0142, **P = 0.0029 and ***P < 0.001 (Student's t-test). (c) Quantitative real-time PCR analysis of drl.3 in whole embryos at the indicated ages. The relative expression of drl.3 was normalized to the expression of gapdh. (d–e) WISH of drl.2 (d) and drl.3 (e) during embryonic development. Ages of embryos are indicated. Embryos at 2-cell and 30% epiboly stages are shown in a lateral view, animal pole at the top. 50% epiboly embryos are shown in an animal pole view, dorsal to the right. Embryos at 75% and 90% epiboly are shown as lateral views, dorsal to the right. Staged embryos at 10 somites, 18 somites, 24 and 48 hpf are shown as lateral views, anterior to the left. White arrows indicate ALM; asterisks indicate ICM; black arrows indicate PLM; arrowheads indicate AGM cells. Insets show magnified AGM region of corresponding embryo. (f) RT-PCR analysis of the drl family members and β−actin from purified populations of hematopoietic cells. The age of the embryos and the transgenic lines from which cells were purified are indicated. (g) Quantitative real-time PCR analysis of drl.3 in 24 hpf sorted hematopoietic cells. The relative expression of drl.3 was normalized to the expression of gapdh. ***P < 0.0001 (Student's t-test). (b–c, g) Bars show mean ± S.D. (a–b, f) Full-length gel images are provided in Supplemental Figure 8.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
drl AB standard conditions 1-cell Not Detected whole organism RTPCR
1k-cell Not Detected whole organism RTPCR
30%-epiboly whole organism RTPCR
90%-epiboly whole organism RTPCR
1-4 somites whole organism RTPCR
14-19 somites whole organism RTPCR
20-25 somites whole organism RTPCR
Prim-5 whole organism RTPCR
Long-pec whole organism RTPCR
Day 5 whole organism RTPCR
cz2Tg standard conditions Day 5 lymphoid progenitor cell RTPCR
la2Tg standard conditions Long-pec thrombocyte RTPCR
Day 5 thrombocyte RTPCR
sd2Tg standard conditions Prim-5 erythroid progenitor cell RTPCR
Long-pec erythroid progenitor cell RTPCR
Day 5 erythroid progenitor cell RTPCR
uwm1Tg standard conditions Prim-5 myeloid cell RTPCR
Long-pec myeloid cell RTPCR
Day 5 myeloid cell RTPCR
y1Tg standard conditions 1-4 somites endothelial cell RTPCR
14-19 somites endothelial cell RTPCR
Prim-5 endothelial cell RTPCR
Long-pec endothelial cell RTPCR
Day 5 endothelial cell RTPCR
zdf11Tg standard conditions 14-19 somites common myeloid progenitor RTPCR
Prim-5 common myeloid progenitor RTPCR
Long-pec common myeloid progenitor RTPCR
Day 5 common myeloid progenitor RTPCR
zdf8Tg standard conditions Day 5 lymphoid progenitor cell RTPCR
drll.1 AB standard conditions 1-cell Not Detected whole organism RTPCR
1k-cell Not Detected whole organism RTPCR
30%-epiboly whole organism RTPCR
90%-epiboly whole organism RTPCR
1-4 somites whole organism RTPCR
14-19 somites whole organism RTPCR
20-25 somites whole organism RTPCR
Prim-5 whole organism RTPCR
Long-pec whole organism RTPCR
Day 5 whole organism RTPCR
cz2Tg standard conditions Day 5 lymphoid progenitor cell RTPCR
la2Tg standard conditions Long-pec thrombocyte RTPCR
Day 5 Not Detected thrombocyte RTPCR
sd2Tg standard conditions Prim-5 erythroid progenitor cell RTPCR
Long-pec erythroid progenitor cell RTPCR
Day 5 erythroid progenitor cell RTPCR
uwm1Tg standard conditions Prim-5 myeloid cell RTPCR
Long-pec myeloid cell RTPCR
Day 5 myeloid cell RTPCR
y1Tg standard conditions 1-4 somites endothelial cell RTPCR
14-19 somites endothelial cell RTPCR
Prim-5 endothelial cell RTPCR
Long-pec endothelial cell RTPCR
Day 5 endothelial cell RTPCR
zdf11Tg standard conditions 14-19 somites common myeloid progenitor RTPCR
Prim-5 common myeloid progenitor RTPCR
Long-pec common myeloid progenitor RTPCR
Day 5 common myeloid progenitor RTPCR
zdf8Tg standard conditions Day 5 lymphoid progenitor cell RTPCR
drll.2 AB standard conditions 1-cell Not Detected whole organism RTPCR
2-cell Not Detected whole organism ISH
1k-cell Not Detected whole organism RTPCR
50%-epiboly presumptive mesoderm ISH
30%-epiboly whole organism ISH
30%-epiboly whole organism RTPCR
75%-epiboly mesoderm ISH
90%-epiboly mesoderm ISH
90%-epiboly whole organism RTPCR
1-4 somites whole organism RTPCR
10-13 somites anterior lateral mesoderm ISH
14-19 somites anterior lateral mesoderm ISH
14-19 somites intermediate cell mass of mesoderm ISH
14-19 somites whole organism RTPCR
20-25 somites whole organism RTPCR
Prim-5 intermediate cell mass of mesoderm ISH
Prim-5 posterior lateral mesoderm ISH
Prim-5 whole organism RTPCR
Long-pec erythroid lineage cell ISH
Long-pec ventral wall of dorsal aorta ISH
Long-pec whole organism RTPCR
Day 5 whole organism RTPCR
cz2Tg standard conditions Day 5 lymphoid progenitor cell RTPCR
la2Tg standard conditions Long-pec thrombocyte RTPCR
Day 5 thrombocyte RTPCR
sd2Tg standard conditions Prim-5 erythroid progenitor cell RTPCR
Long-pec erythroid progenitor cell RTPCR
Day 5 erythroid progenitor cell RTPCR
uwm1Tg standard conditions Prim-5 myeloid cell RTPCR
Long-pec myeloid cell RTPCR
Day 5 myeloid cell RTPCR
y1Tg standard conditions 1-4 somites endothelial cell RTPCR
14-19 somites endothelial cell RTPCR
Prim-5 endothelial cell RTPCR
Long-pec endothelial cell RTPCR
Day 5 endothelial cell RTPCR
zdf11Tg standard conditions 14-19 somites common myeloid progenitor RTPCR
Prim-5 common myeloid progenitor RTPCR
Long-pec common myeloid progenitor RTPCR
Day 5 common myeloid progenitor RTPCR
zdf8Tg standard conditions Day 5 lymphoid progenitor cell RTPCR
drll.3 AB standard conditions 2-cell Not Detected whole organism ISH
1-cell Not Detected whole organism RTPCR
1k-cell Not Detected whole organism RTPCR
50%-epiboly presumptive mesoderm ISH
30%-epiboly whole organism ISH
30%-epiboly whole organism RTPCR
75%-epiboly mesoderm ISH
90%-epiboly mesoderm ISH
90%-epiboly whole organism RTPCR
1-4 somites whole organism RTPCR
10-13 somites anterior lateral mesoderm ISH
14-19 somites anterior lateral mesoderm ISH
14-19 somites intermediate cell mass of mesoderm ISH
14-19 somites whole organism RTPCR
20-25 somites whole organism RTPCR
Prim-5 intermediate cell mass of mesoderm ISH
Prim-5 posterior lateral mesoderm ISH
Prim-5 whole organism RTPCR
Long-pec erythroid lineage cell ISH
Long-pec ventral wall of dorsal aorta ISH
Long-pec whole organism RTPCR
Day 5 whole organism RTPCR
cz2Tg standard conditions Day 5 lymphoid progenitor cell RTPCR
la2Tg standard conditions Long-pec thrombocyte RTPCR
Day 5 thrombocyte RTPCR
sd2Tg standard conditions Prim-5 erythroid progenitor cell RTPCR
Long-pec erythroid progenitor cell RTPCR
Day 5 erythroid progenitor cell RTPCR
uwm1Tg standard conditions Prim-5 myeloid cell RTPCR
Long-pec myeloid cell RTPCR
Day 5 myeloid cell RTPCR
y1Tg standard conditions 1-4 somites endothelial cell RTPCR
14-19 somites endothelial cell RTPCR
Prim-5 endothelial cell RTPCR
Long-pec endothelial cell RTPCR
Day 5 endothelial cell RTPCR
zdf11Tg standard conditions 14-19 somites common myeloid progenitor RTPCR
Prim-5 common myeloid progenitor RTPCR
Long-pec common myeloid progenitor RTPCR
Day 5 common myeloid progenitor RTPCR
zdf8Tg standard conditions Day 5 lymphoid progenitor cell RTPCR
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Scientific Reports for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Sci. Rep.